Permanent draft genome sequences of cadmium-resistant isolates of Cupriavidus from soils within the Tar Creek Superfund site

Abstract

Soil samples taken near the abandoned town of Picher, OK, USA, were used to enrich and isolate bacteria in the presence of cadmium. Isolates reported belong to the genus Cupriavidus. Here, we report their permanent draft sequences with an emphasis on genes conferring resistance to cadmium.

Keywords: Cupriavidus; Tar Creek Superfund site; cadmium; cobalt; czcCBA; lead; zinc.

Fig 1

Maximum likelihood phylogeny of 16S rRNA genes. Mega11 (17) was used to align sequences using MUSCLE (18) and identify GTRGAMMA+I as the optimal substitution model. The phylogeny was created in RAxML (11) with 1,000 bootstrap replicates. All validly named species of Cupriavidus were included. Sequenced genomes are shown in green text, type strains without genome sequences are shown in black text and our strains are depicted in blue text. NCBI (alphanumeric) or IMG accession numbers (numeric) are listed in parentheses. When our strains’ sequences were found to be identical to one another (e.g., EM19 and EM23), one was removed from the alignment for construction of the phylogeny, as suggested by RAxML documentation (11). Subsequently, these names were added to the tree during annotation. Bootstrap support below 65% was omitted from the tree. Scale bar represents the nucleotide substitutions per site.