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. 2024 Nov 26.
doi: 10.1007/s12602-024-10414-0. Online ahead of print.

Postbiotic Effect of Escherichia coli CEC15 and Escherichia coli Nissle 1917 on a Murine Model of 5-FU-induced Intestinal Mucositis

Rafael de Assis Glória #  1 , Tales Fernando da Silva #  1   2 , Tomás Andrade Magalhães Gomes  1 , Kátia Duarte Vital  3 , Simone Odília Antunes Fernandes  3 , Valbert Nascimento Cardoso  3 , Ênio Ferreira  4 , Jean-Mark Chatel  5 , Philippe Langella  5 , Claire Cherbuy  5 , Yves Le Loir  2 , Gwénaël Jan  2 , Éric Guédon  2 , Vasco Ariston de Carvalho Azevedo  6
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Free article

Postbiotic Effect of Escherichia coli CEC15 and Escherichia coli Nissle 1917 on a Murine Model of 5-FU-induced Intestinal Mucositis

Rafael de Assis Glória et al. Probiotics Antimicrob Proteins. .
Free article

Abstract

Probiotics are live microorganisms that, when administered in adequate amounts, can bring health benefits to the host. Most of these organisms are found naturally in the human gastrointestinal tract. Escherichia coli strains Nissle 1917 (EcN), and CEC15 have shown beneficial effects in murine models of intestinal inflammation, such as colitis and mucositis. The present study evaluated the effects as postbiotic of heat-inactivated and cell-free supernatant preparations of EcN and CEC15 in attenuating 5-fluorouracil (5-FU)-induced intestinal mucositis in mice and compared them with the probiotic effects of the live preparations. BALB/c mice were fed, by daily gavage, with 1010 CFU of live or inactivated bacteria or with 300 µL of cell-free supernatant for 12 days. On the 10th day, all animals, except for the control group, received an intraperitoneal injection of 5-FU (300 mg/kg). After 72 h of 5-FU administration, animals were euthanized, and the ileum and blood were collected for analysis. Treatments with live and heat-inactivated CEC15 mitigated weight loss, preserved intestinal length, reduced histological damage, maintained goblet cells, decreased neutrophil infiltration, and modulated expression of inflammatory and barrier genes when compared to 5-FU mucositis controls. EcN showed more limited effects. CEC15 upregulated mRNA expression of the mucin MUC2 and tight junction protein TJP1. CEC15 demonstrated protective effects against 5-FU-induced mucositis, whether administered with live, heat-inactivated, or cell-free supernatant. This suggests that CEC15 mediates a protective response via secreted metabolites and does not require viability. The postbiotic forms of CEC15 present advantages for use in immunocompromised patients. This study elucidates the anti-inflammatory and barrier-protective effects of CEC15 against intestinal mucositis.

Keywords: Escherichia coli CEC15; Escherichia coli Nissle 1917; Mucositis; Paraprobiotics; Postbiotics; Probiotics.

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Conflict of interest statement

Declarations. Ethics Approval and Consent to Participate: The experiment was conducted with the Brazilian College of Animal Experimentation (COBEA) and approved by the “Use of Animals Ethics Committee” from UFMG (CEUA—UFMG) under protocol 210/2022. Competing Interests: The authors declare no competing interests.

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