Detection and quantification of lactoferrin: Innovations, applications, and challenges

Abstract

Lactoferrin (LF), is an 80 kDa glycoprotein with diverse bio-functions in the human body. It is involved in host defense, tumor growth inhibition, anti-inflammatory and enzymatic activity, and demonstrates antioxidant, antimicrobial, antiviral, and antiparasitic properties. As part of the transferrin family, LF is recognized as an iron-binding glycoprotein. Reliable analytical methods are crucial for ensuring consumer safety and enhancing food production through hazard analysis and good manufacturing practices. This review details recent advancements in analytical methods, offering an in-depth look at their primary applications for LF detection. The article underscores the advantages and disadvantages of common analytical techniques, such as conventional and modern methods. Additionally, it explores developments in biosensors related to detection mechanisms, including the use of nanomaterials as signal probes and carriers for loading signal probes; also addressing sensitivity and performance evaluation. Finally, it considers the challenges and potential opportunities in the advancement of biosensors for LF detection.

Keywords: Biosensors; Electrochemical; Food; Lactoferrin; Optical.