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. 2024 Dec 17;13(12):1686-1690.
doi: 10.1021/acsmacrolett.4c00656. Epub 2024 Nov 26.

A Macrocycle-Mediated Protein Cage

Ronan J Flood  1 , Aurélien Thureau  2 , Peter B Crowley  1
Affiliations

A Macrocycle-Mediated Protein Cage

Ronan J Flood et al. ACS Macro Lett. .

Abstract

Engineered protein cages are of great interest considering their diverse applications in delivery and catalysis. Here, we describe macrocycle-triggered icosahedral cage assembly of a designed β-propeller. Cage assembly was evidenced by small-angle X-ray scattering and X-ray crystallography.

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Conflict of interest statement

The authors declare no competing financial interest.

Figures

Figure 1
Figure 1
Surface and sphere representations of Pent and sclx8 (an extended conformation with carbon, sulfur, and oxygen in blue, yellow and red) drawn to scale and representative cocrystals of Pent with (A,B) sclx8 or (C) sclx6. The scale bar is 100 μm.
Figure 2
Figure 2
Pent–sclx8I23 unit cell, with proteins shown as gray surfaces. A 3-fold rotational axis is highlighted in dark gray. The unit cell contains two copies of the cage, with one complete cage in the center and four quarters of the cage arranged on the corners. The unit cell length is 135 Å.
Figure 3
Figure 3
Details of two Pent–sclx6–Pent crystallographic interfaces, highlighting the calixarene glue activity at Lys33. Proteins shown as Cα trace and transparent surfaces with interfacing side chains as sticks. Symmetry mates indicated in dark gray.
Figure 4
Figure 4
Logarithmic SAXS profiles (orange) of 2 mM Pent with (A) 0, (B) 2, or (C) 20 mM sclx8. Blue curves are the CRYSOL-computed scattering of (A) Pent, (B) a Pent dimer (PDB 8R3C), and (C) the icosahedral assembly (Figure 2).
See this image and copyright information in PMC

References

    1. Aumiller W. M.; Uchida M.; Douglas T. Protein Cage Assembly across Multiple Length Scales. Chem. Soc. Rev. 2018, 47, 3433–3469. 10.1039/C7CS00818J. - DOI - PMC - PubMed
    1. Edwardson T. G. W.; Levasseur M. D.; Tetter S.; Steinauer A.; Hori M.; Hilvert D. Protein Cages: From Fundamentals to Advanced Applications. Chem. Rev. 2022, 122, 9145–9197. 10.1021/acs.chemrev.1c00877. - DOI - PubMed
    1. Hsia Y.; Bale J. B.; Gonen S.; Shi D.; Sheffler W.; Fong K. K.; Nattermann U.; Xu C.; Huang P. S.; Ravichandran R.; Yi S.; Davis T. N.; Gonen T.; King N. P.; Baker D. Design of a Hyperstable 60-Subunit Protein Icosahedron. Nature 2016, 535, 136–150. 10.1038/nature18010. - DOI - PMC - PubMed
    1. Bale J. B.; Gonen S.; Liu Y.; Sheffler W.; Ellis D.; Thomas C.; Cascio D.; Yeates T. O.; Gonen T.; King N. P.; Baker D. Accurate Design of Megadalton-Scale Two-Component Icosahedral Protein Complexes. Science 2016, 353, 389–394. 10.1126/science.aaf8818. - DOI - PMC - PubMed
    1. Lutz I. D.; Wang S.; Norn C.; Courbet A.; Borst A. J.; Zhao Y. T.; Dosey A.; Cao L.; Xu J.; Leaf E. M.; Treichel C.; Litvicov P.; Li P.; Goodson A. D.; Rivera-Sánchez P.; Bratovianu A. M.; Baek M.; King N. P.; Ruohola-Baker H.; Baker D. Top-down Design of Protein Architectures with Reinforcement Learning. Science 2023, 380, 266–273. 10.1126/science.adf6591. - DOI - PubMed

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