Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Nov 12;14(11):1461.
doi: 10.3390/life14111461.

Characterization of Novel Species of Potassium-Dissolving Purple Nonsulfur Bacteria Isolated from In-Dyked Alluvial Upland Soil for Maize Cultivation

Le Thi My Thu  1 , Ly Ngoc Thanh Xuan  2 , Tran Chi Nhan  2 , Le Thanh Quang  1 , Nguyen Duc Trong  1 , Vo Minh Thuan  1 , Tran Trong Khoi Nguyen  1 , Phan Chi Nguyen  3 , Le Vinh Thuc  1 , Nguyen Quoc Khuong  1
Affiliations

Characterization of Novel Species of Potassium-Dissolving Purple Nonsulfur Bacteria Isolated from In-Dyked Alluvial Upland Soil for Maize Cultivation

Le Thi My Thu et al. Life (Basel). .

Abstract

Potassium (K) is immobilized within the clay minerals, making it unavailable for plant use. Therefore, the current study aimed to (i) select isolates of purple nonsulfur bacteria that can dissolve K (K-PNSB) and (ii) evaluate the production of plant-growth-promoting substances by the K-PNSB isolates. The results revealed that from in-dyked alluvial soils in hybrid maize fields, 61 K-PNSB isolates were obtained under the pH 5.50 conditions. The total dissolved K content (Kdis) by the 61 K-PNSB isolates fluctuated from 56.2 to 98.6 mg L-1. Therein, three isolates, including M-Sl-09, M-So-11, and M-So-14 had Kdis of 48.1-48.8 mg L-1 under aerobic dark condition (ADC) and 47.6-49.7 mg L-1 under microaerobic light condition (MLC). Moreover, these three isolates can also fix nitrogen (19.1-21.5 mg L-1 and 2.64-7.24 mg L-1), solubilize Ca-P (44.3-46.8 mg L-1 and 0.737-6.965 mg L-1), produce indole-3-acetic acid (5.34-7.13 and 2.40-3.23 mg L-1), 5-aminolevulinic acid (1.85-2.39 and 1.53-2.47 mg L-1), siderophores (1.06-1.52 and 0.92-1.26 mg L-1), and exopolymeric substances (18.1-18.8 and 52.0-56.0%), respectively, under ADC and MLC. The bacteria were identified according to their 16S rDNA as Cereibacter sphaeroides M-Sl-09, Rhodopseudomonas thermotolerans M-So-11, and Rhodospeudomonas palustris M-So-14. These potential bacteria should be further investigated as a plant-growth-promoting biofertilizer.

Keywords: alluvial soil in dykes; plant-growth-promoting substances; potassium solubilization; potassium uptake; staple crop.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Nitrogen fixation of potassium-dissolving purple nonsulfur bacteria selected from in-dyked alluvial soil in hybrid maize fields. Note: Different letters indicate a significant difference (p < 0.05).
Figure 2
Figure 2
Ca-P dissolution of potassium-dissolving purple nonsulfur bacteria selected from in-dyked alluvial soil in hybrid maize fields. Note: Different letters indicate a significant difference (p < 0.05).
Figure 3
Figure 3
Production of plant-growth-promoting substances, including (A) indole-3-acetic acid, (B) 5-aminolevulinic acid, (C) exopolymeric substances, and (D) siderophores of the three isolates of potassium-dissolving purple nonsulfur bacteria selected from in-dyked alluvial soil in hybrid maize fields. Note: Different letters indicate a significant difference (p < 0.05). ns: not significant.
Figure 4
Figure 4
Neighbor-joining phylogenetic trees based on 16S rDNA sequences of two selected PNSB strains compared to the closely related strains in the GenBank database. The percentage levels of bootstrap analysis of 1000 replicates are indicated at each node. Bar, 0.2 substitutions per nucleotide position. Access numbers of GenBank sequences are implied in brackets.
Figure 5
Figure 5
Colonies of the selected K-PNSB isolates Cereibacter sphaeroides M-Sl-09, Rhodopseudomonas thermotolerans M-So-11, and Rhodospeudomonas palustris M-So-14 on BIM.
See this image and copyright information in PMC

References

    1. Saboor A., Ali M.A., Hussain S., El Enshasy H.A., Hussain S., Ahmed N., Gafurf A., Sayyed R.Z., Fahad S., Danish S., et al. Zinc nutrition and arbuscular mycorrhizal symbiosis effects on maize (Zea mays L.) growth and productivity. Saudi J. Biol. Sci. 2021;28:6339–6351. doi: 10.1016/j.sjbs.2021.06.096. - DOI - PMC - PubMed
    1. An N.N., Shang N., Zhao X., Tie X.Y., Guo W.B., Li D., Wang L.J., Wang Y. Occurrence, regulation, and emerging detoxification techniques of aflatoxins in maize: A review. Food Rev. Int. 2024;40:92–114. doi: 10.1080/87559129.2022.2158339. - DOI
    1. Food and Agriculture Organization of the United Nations (FAOSTAT) [(accessed on 10 May 2024)]. Available online: http://faostat3.fao.org/home/E.
    1. Crista F., Boldea M., Radulov I., Lato A., Crista L., Dragomir C., Berbecea A., Nita L., Okros A. The impact of chemical fertilization on maize yield. Res. J. Agric. Sci. 2014;40:172–177.
    1. Nasar J., Khan W., Khan M.Z., Gitari H.I., Gbolayori J.F., Moussa A.A., Mandozai A., Rizwan N., Anwari G., Maroof S.M. Photosynthetic activities and photosynthetic nitrogen use efficiency of maize crop under different planting patterns and nitrogen fertilization. J. Soil Sci. Plant Nutr. 2021;21:2274–2284. doi: 10.1007/s42729-021-00520-1. - DOI

LinkOut - more resources