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. 2024 Nov 27;123(11):398.
doi: 10.1007/s00436-024-08407-7.

Identification and Immunological Characterization of Annexin B8 and Annexin E1 from Spirometra Erinaceieuropaei Spargana

Ruijia Fu #  1   2 , Yanquan Xu #  3 , Gang Lu  2 , Fan Zhang  4 , Pei Liang  5   6 , Dayong Wang  7   8
Affiliations

Identification and Immunological Characterization of Annexin B8 and Annexin E1 from Spirometra Erinaceieuropaei Spargana

Ruijia Fu et al. Parasitol Res. .

Abstract

Sparganosis is a parasitic zoonotic disease that poses a serious threat to public hygiene and human health. Annexin is a phospholipid-binding protein with calcium ion binding activity, serving various important functions, including interaction with the parasite and regulation of the host's immune response. In this study, two annexin (ANX) family genes, Spirometra erinaceieuropaei (S. erinaceieuropaei) Annexin B8 (SeANXB8) and E1 (SeANXE1), isolated from spargana, were cloned and immunologically characterized. Both recombinant S. erinaceieuropaei Annexin B8 (rSeANXB8) and E1 (rSeANXE1) were specifically recognized by serum from rats immunized with the recombinant proteins, displaying strong immunoreactivity. They are also among the major components of sparganum excretion/secretion products (ESPs). SeANXE1 was identified in the parasite's tegument, testis, genital pore, ovary, and eggs, while SeANXB8 was found in the parasite's tegument and eggs. Plasminogen (PLG)-binding assays revealed that the two annexins could bind to human PLG in a concentration-dependent manner, which was blocked by the corresponding antibodies. These findings suggest that SeANXB8 and SeANXE1 may be involved in host-parasite interaction and may influence the host's immune response during sparganosis. They could be potential diagnosis and vaccination targets for sparganosis.

Keywords: Spirometra erinaceieuropaei; Annexin B8; Annexin E1; Plasminogen-binding protein; Sparganum.

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Conflict of interest statement

Declarations. Ethics approval and consent to participate: The Institutional Animal Care and Use Committee of Hainan University (Hainan, China) authorized the animal experimental protocols, according to the Manipulative Technique for the Care and Use of Laboratory Animals (second revision) established by the State Scientifc and Technological Commission of China. Consent for publication: The manuscript does not contain any individual person’s data in any form (including any individual details, images or videos). All authors have consented and approved the publication of the paper. Competing interests: The authors declare no competing interests.

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