Sewer system sampling for wastewater-based disease surveillance: Is the work worth it?
- PMID: 39611680
- DOI: 10.2166/wh.2024.301
Sewer system sampling for wastewater-based disease surveillance: Is the work worth it?
Abstract
Wastewater treatment plant (WWTP) influent sampling is commonly used in wastewater-based disease surveillance to assess the circulation of pathogens in the population aggregated in a catchment area. However, the signal can be lost within the sewer network due to adsorption, degradation, and dilution processes. The present work aimed to investigate the dynamics of SARS-CoV-2 concentration in three sub-catchments of the sewer system in the city of Hildesheim, Germany, characterised by different levels of urbanisation and presence/absence of industry, and to evaluate the benefit of sub-catchment sampling compared to WWTP influent sampling. Our study shows that sampling and analysis of virus concentrations in sub-catchments with particular settlement structures allows the identification of high concentrations of the virus at a local level in the wastewater, which are lower in samples collected at the inlet of the treatment plant covering the whole catchment. Higher virus concentrations per inhabitant were found in the sub-catchments in comparison to the inlet of the WWTP. Additionally, sewer sampling provides spatially resolved concentrations of SARS-CoV-2 in the catchment area, which is important for detecting local high incidences of COVID-19.
Keywords: SARS-CoV-2; digital droplet PCR; sewer system sampling; wastewater-based disease surveillance.
© 2024 The Authors This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC BY 4.0), which permits copying, adaptation and redistribution, provided the original work is properly cited (http://creativecommons.org/licenses/by/4.0/).
Conflict of interest statement
The authors declare there is no conflict.
Erratum for
-
Corrigendum: Journal of Water and Health, 22 (11): 2218-2232: Sewer system sampling for wastewater-based disease surveillance: Is the work worth it? Urda Düker, Regina Nogueira, Estefania Carpio-Vallejo, Ingeborg Joost, Katharina Hüppe, Roland Suchenwirth, Yvonne Saathoff and Markus Wallner, https://dx.doi.org/10.2166/wh.2024.301.J Water Health. 2025 Apr;23(4):561-562. doi: 10.2166/wh.2025.002. J Water Health. 2025. PMID: 40298272 No abstract available.
References
-
- Ahmed W., Angel N., Edson J., Bibby K., Bivins A., O'Brien J. W., Choi P. M., Kitajima M., Simpson S. L., Li J., Tscharke B., Verhagen R., Smith W. J. M., Zaugg J., Dierens L., Hugenholtz P., Thomas K. V. & Mueller J. F. (2020) First confirmed detection of SARS-CoV-2 in untreated wastewater in Australia: A proof of concept for the wastewater surveillance of COVID-19 in the community, The Science of the Total Environment, 728, 138764. https://doi.org/10.1016/j.scitotenv.2020.138764. - PMC - PubMed
-
- Budd W. (1873) Typhoid fever its nature, mode of spreading, and prevention, American Journal of Public Health, 8 (8), 610–612. doi:10.2105/ajph.8.8.610. - PMC
-
- Cameron D. & Jones I. G. (1983) John snow, the broad street pump and modern epidemiology, International Journal of Epidemiology, 12 (4), 393–396. doi:10.1093/ije/12.4.393. - PubMed
-
- Corman V. M., Landt O., Kaiser M., Molenkamp R., Meijer A., Chu D. K., Bleicker T., Brünink S., Schneider J., Schmidt M. L., Mulders D. G., Haagmans B. L., van der Veer B., van den Brink S., Wijsman L., Goderski G., Romette J.-L., Ellis J., Zambon M., Peiris M., Goossens H., Reusken C., Koopmans M. P. & Drosten C. (2020) Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR, Eurosurveillance, 25, 23–30. https://doi.org/10.2807/1560-7917.ES.2020.25.3.2000045. - PMC
-
- Empfehlung (EU) 2021/472 (2021) Empfehlung (EU) 2021/472 der Kommission vom 17. März 2021 über einen gemeinsamen Ansatz zur Einführung einer systematischen Überwachung von SARS-CoV-2 und seinen Varianten im Abwasser in der EU, 3 pp.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Medical
Miscellaneous
