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. 1986 Jan 15;41(2):161-6.
doi: 10.1016/0049-3848(86)90226-4.

A simple one-step HPLC procedure for the purification of the NH2-terminal plasmin-derived B beta 1-42 peptide of human fibrinogen

W F Skogen, G D Wilner

A simple one-step HPLC procedure for the purification of the NH2-terminal plasmin-derived B beta 1-42 peptide of human fibrinogen

W F Skogen et al. Thromb Res. .

Abstract

Simplified procedures were developed to produce and isolate the plasmin-derived B beta 1-42 fragment from human fibrinogen. Peptides generated from fibrinogen by plasmin were separated by reverse phase HPLC chromatography. Two distinct peaks were identified as having amino acid compositions identical to B beta 1-42. The average final yield of HPLC-purified B beta 1-42 was 1.2 mg per 100 mg of fibrinogen. Recovery of the peptide (40-45% when compared to the theoretical yield) was significantly higher than yields obtained by open column techniques demonstrating the advantages of this simple one-step HPLC procedure.

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