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. 2024 Nov 15:15:1496843.
doi: 10.3389/fmicb.2024.1496843. eCollection 2024.

Enhanced biosorption of cadmium ions on immobilized surface-engineered yeast using cadmium-binding peptides

Songting Wang #  1   2 , Yongmei Sun #  1   2 , Shihong Wang  1 , Chunkun Fan  3 , Daojie Wang  1 , Fei Liu  1 , Haiyan Zhang  1   2
Affiliations

Enhanced biosorption of cadmium ions on immobilized surface-engineered yeast using cadmium-binding peptides

Songting Wang et al. Front Microbiol. .

Abstract

A new type of cadmium (Cd) ion cell surface adsorbent was developed by integrating bacteriophage display peptide library technology with cell surface display technology. Cd2+ chelating resin served as the target molecule in screening experiments, leading to the identification of four Cd2+ -binding peptides. These peptides were introduced into Saccharomyces cerevisiae via the pYD1 plasmid using lithium acetate heat shock transformation. Adsorption efficiency tests indicated that the engineered yeasts adsorbed more Cd2+ than the control strain EBY100 when exposed to the same amount of Cd2+. Among these peptides, sequence 3-containing strain was demonstrated to have the highest Cd2+ adsorption efficiency, being 35% higher than the control strain. Additionally, when this recombinant yeast strain was immobilized using sodium alginate, the adsorption efficiency was increased by 55.7% compared to the control strain.

Keywords: biosorption; cadmium-binding peptide; immobilization; phage peptide library; surface-engineered yeast.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Profile of the pYD1 plasmid.
Figure 2
Figure 2
Changes in the P/N values in the three rounds of screening.
Figure 3
Figure 3
Confirmation of successful transformation of S. cerevisiae. M represents DNA Marker, 1 is gE1 PCR fragment, 2 is gE3 PCR fragment, 3 is gE6 PCR fragment, and 4 is gE11 PCR fragment.
Figure 4
Figure 4
Adsorption rate of EBY100 series strains over time.
Figure 5
Figure 5
Immobilized cells after Cd2+ adsorption.
See this image and copyright information in PMC

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