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. 2025:2860:65-82.
doi: 10.1007/978-1-0716-4160-6_5.

Bioinformatics for the Structural Genomics of Poxviruses

Paolo Ribeca  1   2
Affiliations

Bioinformatics for the Structural Genomics of Poxviruses

Paolo Ribeca. Methods Mol Biol. 2025.

Abstract

Poxviruses are large, complex viruses, and their host species are widespread across the tree of life. As a result, the bioinformatics analysis of their genomes can be complex. Here we show how a few helpful tools and strategies can be used to inform the analysis, leading to a better understanding of the structural properties of poxvirus genomes and to a more accurate quality control of, or comparison between, assembled sequences.

Keywords: Assembly; Bioinformatics; Genomics; Mappability; Phylogeny; Poxviruses; Quality control; Repeats; Sequence analysis; Structural analysis.

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References

    1. Amgarten D, Upton C (2018) Bioinformatic approaches for comparative analysis of viruses. In: Setubal JC, Stoye J, Stadler PF (eds) Comparative genomics: methods and protocols. Springer, New York, NY, pp 401–417. https://doi.org/10.1007/978-1-4939-7463-4_15 - DOI
    1. ‘DNA sequencing theory’, Wikipedia. November 07, 2023 Accessed 10 May 2024 [Online]. Available: https://en.wikipedia.org/w/index.php?title=DNA_sequencing_theory
    1. Nurk S et al (2022) The complete sequence of a human genome. Science 376(6588):44–53. https://doi.org/10.1126/science.abj6987 - DOI - PubMed - PMC
    1. Derrien T et al (2012) Fast computation and applications of genome mappability. PLoS One 7(1):e30377. https://doi.org/10.1371/journal.pone.0030377 - DOI - PubMed - PMC
    1. Li D, Liu C-M, Luo R, Sadakane K, Lam T-W (2015) MEGAHIT: an ultra-fast single-node solution for large and complex metagenomics assembly via succinct de Bruijn graph. Bioinformatics 31(10):1674–1676. https://doi.org/10.1093/bioinformatics/btv033 - DOI - PubMed

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