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. 2025:2860:149-156.
doi: 10.1007/978-1-0716-4160-6_10.

Orthopoxvirus Purification for High Containment Laboratories

Laura Hughes  1 , Kimberly Wilkins  2 , Scott Smith  2 , Victoria A Olson  2 , Panayampalli Subbian Satheshkumar  2
Affiliations

Orthopoxvirus Purification for High Containment Laboratories

Laura Hughes et al. Methods Mol Biol. 2025.

Abstract

Purification of large viruses in a high containment laboratory can create unique challenges. Traditional purification methods for large viruses rely on equipment and techniques that are not ideal for high containment work. Poxvirus purification has long relied on the use of Genetron®, a reagent that is no longer available. Here we describe a purification protocol that is effective for semi-purification of orthopoxviruses and suitable for work in high containment laboratories.

Keywords: High containment laboratory; Orthopoxvirus; Poxvirus; Virus purification.

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