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. 2025 Jan:136:156305.
doi: 10.1016/j.phymed.2024.156305. Epub 2024 Nov 28.

Exploring the mechanism of Taohong Siwu Decoction in treating ischemic stroke injury via the circDnajc1/miR-27a-5p/C1qc signaling axis

Yumeng Li  1 , Qingping Ye  1 , Jingjing Li  1 , Lijuan Zhang  1 , Chao Yu  1 , Sujun Xue  1 , Shuangping Li  1 , Xianchun Duan  2 , Daiyin Peng  3
Affiliations

Exploring the mechanism of Taohong Siwu Decoction in treating ischemic stroke injury via the circDnajc1/miR-27a-5p/C1qc signaling axis

Yumeng Li et al. Phytomedicine. 2025 Jan.

Abstract

Background: Ischemic stroke (IS) is the most prevalent type of cerebrovascular disease. Taohong Siwu Decoction (THSWD) has been demonstrated to have neuroprotective benefits during Cerebral Ischemia-Reperfusion Injury (CIRI). Whether THSWD mitigates CIRI by modulating the circDnajc1/miR-27a-5p/C1qc signaling axis is not known.

Objective: Examine how THSWD provides neuroprotective benefits in reducing the damage wrought by IS.

Methods: We employed middle cerebral artery occlusion/reperfusion (MCAO/R) rat model and oxygen glucose deprivation/re-oxygenation (OGD/R) in vitro model. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR), Western blot (WB), and immunofluorescence analyses (IF) were utilized to detect microglial activation markers TMEM119, CD11b, and Iba1, inflammatory mediators CCL2, CCL6, IL1, IL6, IL10, and TNF-α, as well as circDnajc1, miR-27a-5p, C1qc, C3, and C5aR.

Conclusion: Our data suggest that THSWD can mitigate inflammatory response, inhibit microglial activation and neuron apoptosis, and exert neuroprotective effects by regulating the circDnajc1/miR-27a-5p/C1qc signaling axis.

Keywords: C1qc; Inflammatory; Ischemic stroke; Microglial cell; circDnajc1; miR-27a-5p.

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Conflict of interest statement

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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