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. 2024 Dec 5.
doi: 10.1007/s12602-024-10409-x. Online ahead of print.

Unlocking Probiotic Potential: Genomic Insights into Weissella paramesenteroides UFTM 2.6.1

Beatriz Macedo de Oliveira Rocha  1 , Yasmin Neves Vieira Sabino  1 , Thaís Costa de Almeida  1 , Fabio Bignoto Palacio  1 , Isabela Sguilla Rotta  2 , Vanessa Cordeiro Dias  1 , Vânia Lúcia da Silva  1 , Cláudio Galuppo Diniz  1 , Vasco Ariston de Carvalho Azevedo  3 , Bertram Brenig  4 , Siomar de Castro Soares  2 , Aline Dias Paiva  2 , Julliane Dutra Medeiros  1 , Alessandra Barbosa Ferreira Machado  5
Affiliations

Unlocking Probiotic Potential: Genomic Insights into Weissella paramesenteroides UFTM 2.6.1

Beatriz Macedo de Oliveira Rocha et al. Probiotics Antimicrob Proteins. .

Abstract

Weissella, a genus of lactic acid bacteria, has diverse beneficial attributes including probiotic activity and biotechnological applications. Therefore, the investigation of the Weissella genus has garnered growing interest. In this study, we sequenced the complete genome of Weissella paramesenteroides UFTM 2.6.1 isolated from unpasteurized cow's milk from the Triângulo Mineiro region and performed probiogenomic analyses. Taxonomic characterization confirmed the identity of W. paramesenteroides. The genome comprises 1926 protein-coding genes, mainly related to cell metabolism, information storage and processing, and cellular processes and signaling. Ninety-nine unique genes associated with probiotic functions were identified in the genome of W. paramesenteroides UFTM 2.6.1, including genes involved in stress response, bacterial persistence in the gastrointestinal tract, and biosynthesis of vitamins. In silico analysis of bacteriocin-related genes identified Pediocin, and subsequent in vitro testing confirmed that W. paramesenteroides UFTM 2.6.1 exhibits antimicrobial activity against Listeria spp. Genomic characterization revealed the presence of the replicon pLCK4 and four prophage regions, one of which was intact. Moreover, no CRISPR-Cas array or associated Cas proteins were found, along with an absence of resistance and virulence genes, suggesting a safety aspect of the evaluated strain. Pan-genome analysis unveiled 204 exclusive genes in the genome of W. paramesenteroides UFTM 2.6.1, which includes metabolism and stress-associated genes. In general, the results indicate probiotic potential of W. paramesenteroides UFTM 2.6.1. Further studies are required to ensure the safety and beneficial effects of this bacterium in vivo, aiming for future applications in the food industry and animal and human medicine.

Keywords: Weissella; Bioinformatic; Lactic acid bacteria; Probiogenomic; Probiotic; Whole-genome sequencing.

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Conflict of interest statement

Declarations. Competing Interests: The authors declare no competing interests.

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