Evaluation of the persistence of Epichloë endophyte and its possible effect on fungal assemblages in dead leaf sheaths

Abstract

The systemic fungal endophytes of the genus Epichloë inhabit the aerial part of host grasses. Recent studies have reported that Epichloë affects the non-systemic endophytic assemblages in live leaves, but few studies that have demonstrated the occurrence of Epichloë and its effect on fungal assemblages in dead leaves. We proposed a hypothesis that Epichloë decreases from live to dead leaves but affects the non-systemic endophytic assemblages also in dead leaves. To test this hypothesis, we sampled leaf sheaths from four leaf types (live, senescent, attached dead and fallen dead) of two native grass species Elymus racemifer and Elymus tsukushiensis var. transiens in Japan and assessed for fungi by DNA metabarcoding. The occurrence of Epichloë OTU was significantly greater in E. tsukushiensis var. transiens than in E. racemifer and varied significantly between the four-leaf types, with decreased and no detections in attached dead and fallen dead leaves, respectively. The composition of non-systemic endophytic assemblages was also significantly affected by the presence/absence of Epichloë OTU, leaf type, host grass species and their interactions. These results supported our hypothesis and suggested that Epichloë can indirectly lead to the changes in belowground processes such as litter decomposition by affecting saprotrophic fungi in dead leaves.

Keywords: Endophytic fungi; Epichloë; Fungal composition; Fungal richness; Leaf litter; Metabarcoding.