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. 2024 Dec;111(12):e16442.
doi: 10.1002/ajb2.16442. Epub 2024 Dec 7.

Scaling relationships of lamina mass per unit area, mean thickness, and leaf bulk tissue density across nine diverse species

Peijian Shi  1 , Linli Deng  1 , Qinyue Miao  1 , Long Chen  1 , Han Yu  2 , Fuyuan Zhu  2 , Karl J Niklas  3
Affiliations

Scaling relationships of lamina mass per unit area, mean thickness, and leaf bulk tissue density across nine diverse species

Peijian Shi et al. Am J Bot. 2024 Dec.

Abstract

Premise: Although previous studies have reported a positive correlation between leaf dry mass per unit area (LMA) and mean leaf thickness (LT), the LMA versus LT scaling relationship has not been determined due to limited sample sizes, despite its importance in estimating leaf bulk tissue density (mass per unit volume).

Methods: This issue was addressed using between 174 and 185 leaves from each of nine phylogenetically diverse species to investigate the LMA vs. LT scaling relationship. For each leaf, lamina thickness was measured at 12 positions (avoiding midribs and major veins) to calculate LT, and LMA was measured based on leaf area and dry mass measurements. Reduced major axis regression protocols were used to determine the LMA vs. LT scaling exponent (i.e., the slope). Bootstrap percentile methods were used to calculate the 95% confidence intervals of slopes.

Results: A statistically significant LMA vs. LT relationship was found for each species; seven of the nine scaling exponents were significantly greater than unity indicating that LMA (and thus leaf bulk tissue density) disproportionately increased with increasing LT. In addition, the conspecific variation in LMA exceeded the interspecific variation in LMA as a consequence of differences in LT.

Conclusions: These results indicate that empirical measurements of LMA and LT can be used to accurately estimate leaf bulk tissue density, which provides insights into adaptive life-history strategies, conspecific variation, and (with sufficiently large data sets) phylogenetic trends.

Keywords: bulk tissue density; deciduous species; evergreen species; leaf area; leaf dry mass; leaf thickness; scaling theory.

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