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. 2022 Mar 17;60(2):230-239.
doi: 10.32615/ps.2022.007. eCollection 2022.

Chlorophyll measurements in Alstroemeria sp. using SPAD-502 meter and the color space CIE L*a*b*, and its validation in foliar senescence

I Villegas-VeláZquez  1 , H A Zavaleta-Mancera  1 , M L Arévalo-Galarza  2 , J Suarez-Espinosa  3 , C Garcia-Osorio  2 , D Padilla-Chacon  1 , I G Galvan-Escobedo  1 , J F Jimenez-Bremont  4
Affiliations

Chlorophyll measurements in Alstroemeria sp. using SPAD-502 meter and the color space CIE L*a*b*, and its validation in foliar senescence

I Villegas-VeláZquez et al. Photosynthetica. .

Abstract

Our research aimed to study the correlation between the SPAD-502 readings and the color space CIE L*a*b* values in two cultivars of Alstroemeria sp. during leaf senescence and to evaluate the statistical criteria used in the selection of the best fit calibration functions. We demonstrate the importance of the Akaike information criterion and the parsimonious function besides the coefficient of determination. The reliability of the functions was tested by Student's t-test comparison between the chlorophyll (Chl) estimated from SPAD readings and their chemical concentrations. Polynomial and Hoerl function described well the changes in Chl a and total Chl (a+b) during senescence, but calibration functions are required to perform for each cultivar. We demonstrated that CIE L*a*b* system is reliable to estimate SPAD reading at stages of leaf senescence of Alstroemeria sp. and can be used instead of SPAD-502.

Keywords: Akaike; SPAD calibration; color measurements; greenness; ornamentals; photosynthetic pigments.

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Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1. Alstroemeria sp.: (A) leaf and red flowers of ‘Fogo’, (B) leaf and yellow flowers of ‘Tonatiuh’, (C) ‘Fogo’ bud flowers at harvest time, (D) ‘Tonatiuh’ bud flowers at harvest time.
Fig. 2
Fig. 2. (A) Morphology of the inflorescence of Alstroemeria sp. The flowering stem of Alstroemeria sp. is botanically classified as a compound umbel, where a simple umbel is identified as an indeterminate, open flat-topped inflorescence, whose pedicels and peduncles arise from a ‘common point’ (a) and the compound umbel is when each peduncle terminates in a secondary umbel. For the experiment, leaves were counted down from the ‘common point’ to the base of the stem and leaves below the 4th node were removed. (B) Regions (white disks) where the SPAD-502 meter measurements were performed. (C) Regions (white disks) where the color space CIEL*a*b* measurements were performed.
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