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. 2024 Dec 9;82(1):33.
doi: 10.1007/s00284-024-03990-5.

Transcriptome Sequencing of Bacillus sp. TTMP20 and Analysis of the Effect of BDH Gene on Tetramethylpyrazine Synthesis

Shiyu Liang  1 , Mengxiang Ling  1 , Lihua Li  1 , Shanling Gan  2 , Changhua Shang  3
Affiliations

Transcriptome Sequencing of Bacillus sp. TTMP20 and Analysis of the Effect of BDH Gene on Tetramethylpyrazine Synthesis

Shiyu Liang et al. Curr Microbiol. .

Abstract

Bacillus sp. TTMP20 has high tetramethylpyrazine (TTMP) yield. However, the mechanism of TTMP production in this strain is unclear at present, which limits the modification for strain TTMP20. In this study, key metabolic pathways related to TTMP synthesis were identified, which included glycolytic pathway, tricarboxylic acid cycle and nitrogen metabolism pathway. Moreover, transgenic Bacillus sp. TTMP20 with recombinant vector PHY-300PLK-BDH was constructed. 2,3-butanediol dehydrogenase gene (BDH) overexpression considerably reduced TTMP biosynthesis. This study will supply new insight into the regulation of TTMP biosynthesis at key enzyme gene level.

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Conflict of interest statement

Declarations. Conflict of interest: The authors declare that they have no competing interests. Ethical Approval: The study has been conducted under the national regulations. The name of the body is given ethical approval.

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