Enhancing the application of probiotics in probiotic food products from the perspective of improving stress resistance by regulating cell physiological function: A review

Abstract

Probiotic foods are foods containing probiotics, including dairy and non-dairy products, that exert significant beneficial impacts on human health. Benefiting from the rapid progress in systems biology, diverse types of probiotics with prominent health-promoting functionalities are unraveled, albeit such functions could be substantially influenced by the stress environments. Here, we conducted a comprehensive review to characterize the state-of-the-art research on probiotic foods and specific probiotics employed in their production. We summarized the detrimental effects of various environmental stresses, including those encountered during industrial fermentation and storage (in vitro), as well as in vivo conditions such as digestion and intestinal colonization, on the biological functions of probiotics. Furthermore, this review outlines the recent advancements in elucidating the mechanisms of stress resistance, which are expected to enhance targeted probiotic applications and optimize their functional properties. Additionally, we summarized various strategies aimed at improving stress tolerance by regulating cell physiological function, specifically adaptive laboratory evolution, preadaptation treatment, exogenous supplementation, and molecular biological manipulation. This review underscores the significance of enhancing our understanding of stress tolerance mechanisms at a systems level and developing efficacious anti-stress strategies to enhance the application of probiotics while maximizing their biological functionalities.

Keywords: Cell physiological function; Improving strategies; Probiotic foods; Probiotics; Stress resistance.