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. 2024 Oct 30:9:637.
doi: 10.12688/wellcomeopenres.23295.1. eCollection 2024.

The genome sequence of a woodlouse fly, Melanophora roralis (Linnaeus, 1758)

Ryan Mitchell  1 , Olga Sivell  2 ; Natural History Museum Genome Acquisition Lab; Darwin Tree of Life Barcoding collective; Wellcome Sanger Institute Tree of Life Management, Samples and Laboratory team; Wellcome Sanger Institute Scientific Operations: Sequencing Operations; Wellcome Sanger Institute Tree of Life Core Informatics team; Tree of Life Core Informatics collective; Darwin Tree of Life Consortium
Affiliations

The genome sequence of a woodlouse fly, Melanophora roralis (Linnaeus, 1758)

Ryan Mitchell et al. Wellcome Open Res. .

Abstract

We present a genome assembly from an individual female woodlouse fly, Melanophora roralis (Arthropoda; Insecta; Diptera; Rhinophoridae). The genome sequence has a total length of 565.10 megabases. Most of the assembly (98.9%) is scaffolded into 6 chromosomal pseudomolecules, including the X sex chromosome. The mitochondrial genome has also been assembled and is 19.44 kilobases in length. Gene annotation of this assembly on Ensembl identified 20,321 protein-coding genes.

Keywords: Diptera; Melanophora roralis; chromosomal; genome sequence; woodlouse fly.

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Conflict of interest statement

No competing interests were disclosed.

Figures

Figure 1.
Figure 1.. Photograph of the Melanophora roralis (idMelRora1) specimen used for genome sequencing.
Figure 2.
Figure 2.. Genome assembly of Melanophora roralis, idMelRora1.1: metrics.
The BlobToolKit snail plot shows N50 metrics and BUSCO gene completeness. The main plot is divided into 1,000 size-ordered bins around the circumference with each bin representing 0.1% of the 565,136,330 bp assembly. The distribution of scaffold lengths is shown in dark grey with the plot radius scaled to the longest scaffold present in the assembly (128,135,431 bp, shown in red). Orange and pale-orange arcs show the N50 and N90 scaffold lengths (97,632,154 and 85,810,975 bp), respectively. The pale grey spiral shows the cumulative scaffold count on a log scale with white scale lines showing successive orders of magnitude. The blue and pale-blue area around the outside of the plot shows the distribution of GC, AT and N percentages in the same bins as the inner plot. A summary of complete, fragmented, duplicated and missing BUSCO genes in the diptera_odb10 set is shown in the top right. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/GCA_963583895.1/dataset/GCA_963583895.1/snail.
Figure 3.
Figure 3.. Genome assembly of Melanophora roralis: BlobToolKit GC-coverage plot showing sequence coverage (vertical axis) and GC content (horizontal axis).
The circles represent scaffolds, with the size proportional to scaffold length and the colour representing phylum membership. The histograms along the axes display the total length of sequences distributed across different levels of coverage and GC content. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/GCA_963583895.1/dataset/GCA_963583895.1/blob.
Figure 4.
Figure 4.. Genome assembly of Melanophora roralis idMelRora1.1: BlobToolKit cumulative sequence plot.
The grey line shows cumulative length for all scaffolds. Coloured lines show cumulative lengths of scaffolds assigned to each phylum using the buscogenes taxrule. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/GCA_963583895.1/dataset/GCA_963583895.1/cumulative.
Figure 5.
Figure 5.. Genome assembly of Melanophora roralis idMelRora1.1: Hi-C contact map of the idMelRora1.1 assembly, visualised using HiGlass.
Chromosomes are shown in order of size from left to right and top to bottom. An interactive version of this figure may be viewed at https://genome-note-higlass.tol.sanger.ac.uk/l/?d=CWbD9D6wSvWmXBHBkK0qYQ.
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