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Review
. 2024 Jun 5;51(6):430-438.
doi: 10.1159/000538972. eCollection 2024 Dec.

Advancing Transfusion Medicine through Raman Tweezers Spectroscopy: A Review of Recent Progress and Future Perspectives

Mithun Nelliat  1 , Ganesh Mohan  2 , Jijo Lukose  1 , Shamee Shastry  2 , Santhosh Chidangil  1
Affiliations
Review

Advancing Transfusion Medicine through Raman Tweezers Spectroscopy: A Review of Recent Progress and Future Perspectives

Mithun Nelliat et al. Transfus Med Hemother. .

Abstract

Background: Raman tweezers spectroscopy (RTS) is a powerful tool that combines optical tweezers and Raman spectroscopy to study single living cells. RTS has become increasingly popular in biomedical and clinical research due to its high molecular specificity and sensitivity, which enable the study of cell viability, cell deformation, cell-protein, cell-nanoparticle, cell-cell interaction, etc. In transfusion medicine, RTS can give valuable insights into the storage lesions and effects of various preservatives and intravenous fluids on blood cells.

Summary: By analyzing the Raman spectra of individual blood cells, RTS can detect changes in the cellular blood components which can be used to monitor the quality of blood products during storage and transfusion. The present review article highlights the principle and clinical applications of RTS in transfusion medicine.

Key messages: Raman spectroscopy is a versatile analytical method for biomedical research. Combining the Raman spectroscopy method with the optical tweezers technique will allow us to explore the dynamics of live single cells in their physiological medium.

Keywords: Intravenous fluids; Optical tweezers; Raman spectroscopy; Red blood cells; Transfusion.

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Conflict of interest statement

The authors have no conflicts of interest to declare.

Figures

Fig. 1.
Fig. 1.
a An illustration of the Rayleigh and Raman scattering processes (green color arrow pointing up indicates incident light, green color arrow pointing down indicates Rayleigh scattering, red color arrow indicates Stoke’s Raman scattering, and blue color arrow indicates anti-Stoke’s Raman scattering). b Ray diagram representation of optical trapping of a single dielectric sphere in a tightly focused laser beam. a and b represent two rays and Fa, Fb force exerted by a and b; Fscat (scattering force), Fgrad (gradient force).
Fig. 2.
Fig. 2.
Raman spectra of living RBCs in various IV fluids.
Fig. 3.
Fig. 3.
Raman spectra of normal RBC and beta-thalassemic RBC.
See this image and copyright information in PMC

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