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. 1985 Feb;45(2):616-8.

New assay for the O-alkyl cleavage enzyme with alkyl-lysophospholipids as substrates

C Unger, H Eibl, H W von Heyden, G A Nagel
  • PMID: 3967236

New assay for the O-alkyl cleavage enzyme with alkyl-lysophospholipids as substrates

C Unger et al. Cancer Res. 1985 Feb.

Abstract

The O-alkyl cleavage enzyme is important for the metabolism of cytotoxic alkyl-lysophospholipids. We have developed a simple new method for the determination of the enzyme activity which is based on the formation of water-soluble phosphate during the enzyme reaction. This is the first assay which avoids the use of radiolabeled substrates.

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