Neuroendocrine regulatory network underlying physiological shifts between reproduction and aestivation of sea cucumber Apostichopus japonicus: Insights from transcriptome analysis

Abstract

The sea cucumber Apostichopus japonicus is an economically significant marine species in China, with numerous studies focusing on its diverse physiological processes, including seasonal reproduction and aestivation. The neuroendocrine system plays a critical role in regulating these physiological transitions. In this study, we utilized transcriptomic techniques and bioinformatics tools to identify key functional genes in the nerve ring of A. japonicus during four distinct physiological phases: pre-reproduction, post-reproduction, aestivation, and recovery from aestivation. Totally 33 neuropeptide precursors and 521 G protein-coupled receptors (GPCRs) were identified. Highlighted key genes, such as AjCRZP, AjPDFP1b, AjSSP1, AjSSP2, and AjSSTR4, were proposed to contribute to transitions of reproduction to aestivation. The temporal trends and functions of differentially expressed genes (DEGs) were validated through qRT-PCR assays. Additionally, we constructed a preliminary neuroendocrine regulatory network, with AjGALR2 and AjCHRM5 identified as central hub genes. These findings offer valuable insights into the neuroendocrine regulation of reproduction and aestivation in A. japonicus, providing a foundation for further mechanistic studies and enhancing our understanding of sea cucumber biology.

Keywords: Aestivation; Apostichopus japonicus; G protein-coupled receptor; Neuropeptide; Reproduction.