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. 2025 May;55(5):427-429.
doi: 10.1111/cea.14606. Epub 2024 Dec 15.

Detection and Characterisation of Wasp Venom-Specific T Cells Using the ARTE Method in Allergic Patients

Magdalena Kraft  1 , Samira Saleh  1 , Guido Heine  2 , Alexander Scheffold  3 , Petra Bacher  3   4 , Margitta Worm  1
Affiliations

Detection and Characterisation of Wasp Venom-Specific T Cells Using the ARTE Method in Allergic Patients

Magdalena Kraft et al. Clin Exp Allergy. 2025 May.
No abstract available

Keywords: ARTE; IgE; Th2 cells; allergy; anaphylaxis; antigen‐specific T cells; venom.

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Conflict of interest statement

MK reports travel support from ALK‐Abéllo and speaker honoraria from Bencard Allergie GmbH, outside the submitted work. MW received reimbursement for speaker and/or consulting activities from Novartis Pharma GmbH, Sanofi‐Aventis Deutschland GmbH, DBV Technologies S.A, Aimmune Therapeutics UK Limited, Regeneron Pharmaceuticals Inc., Leo Pharma GmbH, Boehringer Ingelheim Pharma GmbH & Co. KG, ALK‐Abelló Arzneimittel GmbH, Lilly Deutschland GmbH, Kymab Limited, Amgen GmbH, AbbVie Deutschland GmbH & Co. KG, Pfizer Pharma GmbH, Mylan Germany GmbH (A Viatris Company), AstraZeneca GmbH, Lilly Deutschland GmbH, GlaxoSmithKline GmbH & Co. KG and Almirall Hermal GmbH. GH reports grants from Deutsche Forschungsgemeinschaft (DFG), during the conduct of the study; personal fees from Allergopharma and speaker Honoria from Abbvie, ALK Abelló, Biotest, Eli Lilly, Leti and Sanofi, outside the submitted work. The rest of the authors declare no relevant conflicts of interest.

Figures

FIGURE 1
FIGURE 1
Wasp venom‐specific IL‐4, IL‐13 and IL‐5‐producing memory T cells can be detected in the blood of allergic donors. (A) Frequencies of venom or MP65 reactive CD154+ T cells among CD4+ PBMCs from wasp venom allergic donors and per cent of patients with detectable levels of these cells before and 1 week after initiation of VIT. Only patients where data regarding both antigens at both time points was available were included in the analysis (n = 19). Values below the detection limit were plotted on the x‐axis under the dotted line for graphical presentation purposes. The frequencies of antigen‐specific T cells were compared using the Wilcoxon test for paired samples, and the percentages of patients with detectable antigen‐specific T cells using Fisher's exact test. ***p < 0.001, **p < 0.01, ns—not significant. (B) Representative example staining for IL4+ CD154+ of CD4+ PBMCs (predated on single live, CD14−, CD20− and CD8− CD4+ lymphocytes) for venom (left panel) positive control ( Candida albicans MP65 antigen; middle panel) and negative control (no antigen during stimulation; right panel) after ARTE enrichment 1 week after initiation of VIT as well as the quantification (percentage) of IL4+, IFNγ+, IL‐2+, IL‐13+ or IL‐5+ or CD45RO+ cells among CD154+ CD4+ PBMCs after stimulation with wasp venom or C. albicans MP65 antigen 1 week after initiation of VIT. Only samples with detectable venom or MP65 antigen‐reactive CD154+ cells were included in the analysis. The line represents the median. Mann–Whitney test. ***p < 0.001, *p < 0.05, ns—not significant.
See this image and copyright information in PMC

References

    1. Bellinghausen I., Metz G., Enk A. H., Christmann S., Knop J., and Saloga J., “Insect Venom Immunotherapy Induces Interleukin‐10 Production and a Th2‐To‐Th1 Shift, and Changes Surface Marker Expression in Venom‐Allergic Subjects,” European Journal of Immunology 27, no. 5 (1997): 1131–1139. - PubMed
    1. Jutel M., Pichler W. J., Skrbic D., Urwyler A., Dahinden C., and Muller U. R., “Bee Venom Immunotherapy Results in Decrease of IL‐4 and IL‐5 and Increase of IFN‐Gamma Secretion in Specific Allergen‐Stimulated T Cell Cultures,” Journal of Immunology 154, no. 8 (1995): 4187–4194. - PubMed
    1. McHugh S. M., Deighton J., Stewart A. G., Lachmann P. J., and Ewan P. W., “Bee Venom Immunotherapy Induces a Shift in Cytokine Responses From a TH‐2 to a TH‐1 Dominant Pattern: Comparison of Rush and Conventional Immunotherapy,” Clinical and Experimental Allergy 25, no. 9 (1995): 828–838. - PubMed
    1. Subramaniam S., Aslam A., Misbah S. A., et al., “Elevated and Cross‐Responsive CD1a‐Reactive T Cells in Bee and Wasp Venom Allergic Individuals,” European Journal of Immunology 46, no. 1 (2016): 242–252. - PMC - PubMed
    1. Bacher P., Schink C., Teutschbein J., et al., “Antigen‐Reactive T Cell Enrichment for Direct, High‐Resolution Analysis of the Human Naive and Memory Th Cell Repertoire,” Journal of Immunology 190, no. 8 (2013): 3967–3976. - PubMed

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