Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2024 Jan-Dec;16(1):2405547.
doi: 10.1080/19490976.2024.2405547. Epub 2024 Oct 7.

Comparative analysis of the duodenojejunal microbiome with the oral and fecal microbiomes reveals its stronger association with obesity and nutrition

Emilie Steinbach  1 , Eugeni Belda  1   2 , Rohia Alili  1 , Solia Adriouch  1 , Charlène J G Dauriat  3   4 , Gianfranco Donatelli  1   5 , Jean-Loup Dumont  5 , Filippo Pacini  5 , Thierry Tuszynski  5 , Véronique Pelloux  1 , Flavien Jacques  1 , Laura Creusot  6   7 , Emavieve Coles  1 , Paul Taillandier  1 , Marta Vazquez Gomez  1 , Davide Masi  1   8 , Véronique Mateo  1 , Sébastien André  1 , Melissa Kordahi  3   4 , Christine Rouault  1 , Jean-Daniel Zucker  1   2 , Harry Sokol  6   7   9 , Laurent Genser  1   10 , Benoit Chassaing  3   4 , Tiphaine Le Roy  1 , Karine Clément  1   11
Affiliations
Comparative Study

Comparative analysis of the duodenojejunal microbiome with the oral and fecal microbiomes reveals its stronger association with obesity and nutrition

Emilie Steinbach et al. Gut Microbes. 2024 Jan-Dec.

Abstract

The intestinal microbiota is increasingly recognized as a crucial player in the development and maintenance of various chronic conditions, including obesity and associated metabolic diseases. While most research focuses on the fecal microbiota due to its easier accessibility, the small intestine, as a major site for nutrient sensing and absorption, warrants further investigation to determine its microbiota composition and functions. Here, we conducted a clinical research project in 30 age- and sex-matched participants with (n = 15) and without (n = 15) obesity. Duodenojejunal fluid was obtained by aspiration during endoscopy. Phenotyping included clinical variables related to metabolic status, lifestyle, and psychosocial factors using validated questionnaires. We performed metagenomic analyses of the oral, duodenojejunal, and fecal microbiome, alongside metabolomic data from duodenojejunal fluid and feces, integrating these data with clinical and lifestyle information. Our results highlight significant associations between duodenojejunal microbiota composition and usual dietary intake, as well as clinical phenotypes, with larger effect sizes than the associations between these variables and fecal microbiota. Notably, we found that the duodenojejunal microbiota of patients with obesity exhibited higher diversity and showed distinct differences in the abundance of several duodenojejunal microbiota species compared with individuals without obesity. Our findings support the relevance of studying the role of the small intestinal microbiota in the pathogenesis of nutrition-related diseases.

Keywords: Small intestine microbiota; dietary quality; duodenojejunal microbiome; metabolic diseases; metabolomics; microbiome; microbiota; nutrition; obesity; oral microbiome.

PubMed Disclaimer

Conflict of interest statement

No potential conflict of interest was reported by the author(s).

Figures

Figure 1.
Figure 1.
Microbiome patterns across three ecosystems of the digestive tract.
Figure 2.
Figure 2.
Integrated compositional landscape of human gut ecosystems from JeMiMe and Shalon et al. studies.
Figure 3.
Figure 3.
Oral, duodenojejunal microbiome and metabolome structure in obesity and their association with lifestyle and clinical phenotype.
Figure 4.
Figure 4.
Alterations of the oral, duodenojejunal microbiome and metabolome composition in obesity and their association with lifestyle and clinical phenotype.
See this image and copyright information in PMC

References

    1. Phelps NH, Singleton RK, Zhou B, Heap RA, Mishra A, Bennett JE, Paciorek CJ, Lhoste VP, Carrillo-Larco RM, Stevens GA, et al. Worldwide trends in underweight and obesity from 1990 to 2022: a pooled analysis of 3663 population-representative studies with 222 million children, adolescents, and adults. Lancet. 2024;403(10431):1027–19. doi:10.1016/S0140-6736(23)02750-2. - DOI - PMC - PubMed
    1. Fan Y, Pedersen O.. Gut microbiota in human metabolic health and disease. Nat Rev Microbiol. 2021;19(1):55–71. doi:10.1038/s41579-020-0433-9. - DOI - PubMed
    1. Aron-Wisnewsky J, Prifti E, Belda E, Ichou F, Kayser BD, Dao MC, Verger EO, Hedjazi L, Bouillot JL, Chevallier JM, et al. Major microbiota dysbiosis in severe obesity: fate after bariatric surgery. Gut. 2019;68(1):70. doi:10.1136/gutjnl-2018-316103. - DOI - PMC - PubMed
    1. Delbaere K, Roegiers I, Bron A, Durif C, Van de Wiele T, Blanquet-Diot S, Marinelli L. The small intestine: dining table of host–microbiota meetings. FEMS Microbiol Rev. 2023;47(3):fuad022. doi:10.1093/femsre/fuad022. - DOI - PMC - PubMed
    1. Steinbach E, Masi D, Ribeiro A, Serradas P, Le Roy T, Clément K. Upper small intestine microbiome in obesity and related metabolic disorders: a new field of investigation. Metabolism. 2023;150:155712. doi:10.1016/j.metabol.2023.155712. - DOI - PubMed

Publication types