Genetic and physical map of the 2,4-dichlorophenoxyacetic acid-degradative plasmid pJP4
- PMID: 3968031
- PMCID: PMC214900
- DOI: 10.1128/jb.161.1.466-468.1985
Genetic and physical map of the 2,4-dichlorophenoxyacetic acid-degradative plasmid pJP4
Abstract
Plasmid pJP4 is an 80-kilobase, IncP1, broad-host-range conjugative plasmid of Alcaligenes eutrophus encoding resistance to mercuric chloride and phenyl mercury acetate and degradation of 2,4-dichlorophenoxyacetic acid, 2-methyl-4-chlorophenoxyacetic acid, and 3-chlorobenzoate. By the use of cloning, transposon mutagenesis, and restriction endonuclease analysis, a biophysical and genetic map of pJP4 was generated.
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