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. 1985 Jan;38(1):82-5.
doi: 10.1136/jcp.38.1.82.

Gas chromatographic identification of Clostridium difficile and detection of cytotoxin from a modified selective medium

P N Levett, K D Phillips

Gas chromatographic identification of Clostridium difficile and detection of cytotoxin from a modified selective medium

P N Levett et al. J Clin Pathol. 1985 Jan.

Abstract

A modification of an existing selective medium for Clostridium difficile is described. Inclusion in the medium of DL nor-leucine and p-hydroxyphenylacetic acid enables identification of C difficile to be made directly from primary isolation plates by gas chromatographic detection of caproic acid and p-cresol. Plugs of agar withdrawn from the selective medium also allow the detection of cytotoxin production in vitro.

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