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. 2024 Nov 22;25(23):12539.
doi: 10.3390/ijms252312539.

Genome-Wide Analysis of BURP Domain-Containing Gene Family in Solanum lycopersicum and Functional Analysis of SlRD1 Under Drought and Salt Stresses

Huiru Sun  1   2 , Jinyu Yang  1 , Bei Fan  1 , Min Ren  1 , Yanfeng Wang  1   2 , Guoliang Chen  1   2 , Guoting Cheng  1   2
Affiliations

Genome-Wide Analysis of BURP Domain-Containing Gene Family in Solanum lycopersicum and Functional Analysis of SlRD1 Under Drought and Salt Stresses

Huiru Sun et al. Int J Mol Sci. .

Abstract

The BURP domain-containing (BURP) genes belong to plant-specific families and are known as essential for various biological processes in plants. However, knowledge of the functions of BURP genes in tomato (Solanum lycopersicum) is lacking. In our study, bioinformatics analysis was performed for the SlBURP gene family, including phylogeny, chromosomal localization, gene structure, cis-acting elements and expression. In addition, the function of SlRD1 in drought and salt stresses was explored. In tomato, fourteen BURP family members were identified, located on five chromosomes, including two tandem duplication clusters. These BURP members were classified into four subfamilies. The promoter regions of SlBURPs harbored numerous hormone- and stress-response elements. Tissue expression analysis showed that several SlBURPs were highly expressed in roots, flowers or fruits. Meanwhile, the expressions of most SlBURPs could be regulated by drought, salt and cold treatments, and some of them also responded to ABA treatment. Moreover, the ectopic expression of SlRD1 in Arabidopsis enhanced tolerances to drought and salt stresses and increased the sensitivity of seed germination to ABA. In conclusion, the comprehensive analysis of the SlBURP family in tomato and the functional exploration of SlRD1 in drought and salt stresses provide a basis for further dissecting the roles of tomato BURP genes.

Keywords: BURP domain-containing genes; SlRD1; drought; salt; tomato.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Phylogenetic trees of BURP proteins in different species. Arabidopsis thaliana, Oryza sativa, Zea mays, Gossypium hirsutum, Vicia faba and Solanum lycopersicum are labeled as At, Os, Zm, Gh, Vf and Sl, respectively. The inserted phylogenetic tree was constructed using BURP proteins from tomato and Arabidopsis. Distinct color sections represent eight subfamilies.
Figure 2
Figure 2
Chromosomal location of SlBURPs. The blue boxes represent tandem duplicated genes.
Figure 3
Figure 3
Phylogenetic tree (A), gene structure (B) and conserved motif (C) analysis of SlBURP family.
Figure 4
Figure 4
Analysis of cis-acting elements in SlBURP promoters. (A) The statistics of different cis-acting elements in the promoters of SlBURP family genes. (B) The distribution of different cis-acting elements in SlBURP promoters. SUM: the total number of cis-acting elements in SlBURP promoters; COUNT: the number of SlBURPs whose promoter contains the respective elements; different colors represent different element types.
Figure 5
Figure 5
Expressions of SlBURPs in different tomato tissues. The numerical values on the right represent the log2 normalization of qRT-PCR values.
Figure 6
Figure 6
Expressions of 8 SlBURPs in tomato leaves after drought (A), salt (200 mM and 400 mM) (B) and cold (4 °C) treatments (C). The asterisks represent significant differences in expressions compared with the control (0 h) based on t-test (* p < 0.05, ** p < 0.01).
Figure 7
Figure 7
Expressions of 8 SlBURPs in tomato leaves after ABA treatment. The asterisks represent significant differences expressions compared with the control (0 h) based on t-test (* p < 0.05, ** p < 0.01).
Figure 8
Figure 8
Phenotypic analysis of ectopically expressed SlRD1 Arabidopsis lines under drought and salt stresses. (A) Phenotypes of transgenic Arabidopsis lines and WT under 100 mM mannitol and 100 mM NaCl treatments. (B) The survival rates of transgenic Arabidopsis lines and WT under mannitol and NaCl treatments. (C) The primary root lengths of transgenic Arabidopsis lines and WT under control, 100 mM mannitol and 100 mM NaCl treatments. WT, wild type. CK, control. The asterisks represent significant differences compared with the control based on t-test (* p < 0.05, ** p < 0.01).
Figure 9
Figure 9
Seed germination of ectopically expressed SlRD1 Arabidopsis lines and WT under control and 10 μM ABA treatment. (A) Seed germination phenotypes of transgenic Arabidopsis lines and WT grown on 1/2 MS media and 1/2 MS media with 10 μM ABA for 5 days. (B) The seed germination rates of transgenic Arabidopsis lines and WT grown on 1/2 MS media from 0 days to 7 days. (C) The seed germination rates of transgenic Arabidopsis lines and WT grown on 1/2 MS media with 10 μM ABA from 0 day to 7 days. The asterisks represent significant differences compared with the control based on t-test (** p < 0.01).
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References

    1. Albacete A., Ghanem M.E., Martínez-Andújar C., Acosta M., Sánchez-Bravo J., Martínez V., Lutts S., Dodd I.C., Pérez-Alfocea F. Hormonal changes in relation to biomass partitioning and shoot growth impairment in salinized tomato (Solanum lycopersicum L.) plants. J Exp. Bot. 2008;59:4119–4131. doi: 10.1093/jxb/ern251. - DOI - PMC - PubMed
    1. Liang Y., Ma F., Li B., Guo C., Hu T., Zhang M., Liang Y., Zhu J., Zhan X. A bHLH transcription factor, SlbHLH96, promotes drought tolerance in tomato. Hortic. Res. 2022;9:uhac198. doi: 10.1093/hr/uhac198. - DOI - PMC - PubMed
    1. Yu S., Yang F., Zou Y., Yang Y., Li T., Chen S., Wang Y., Xu K., Xia H., Luo L. Overexpressing PpBURP2 in rice increases plant defense to abiotic stress and bacterial leaf blight. Front. Plant. Sci. 2022;13:812279. doi: 10.3389/fpls.2022.812279. - DOI - PMC - PubMed
    1. Liu H., Ma Y., Chen N., Guo S., Liu H., Guo X., Chong K., Xu Y. Overexpression of stress-inducible OsBURP16, the β subunit of polygalacturonase 1, decreases pectin content and cell adhesion and increases abiotic stress sensitivity in rice. Plant Cell Environ. 2014;37:1144–1158. doi: 10.1111/pce.12223. - DOI - PMC - PubMed
    1. Harshavardhan V.T., Van Son L., Seiler C., Junker A., Weigelt-Fischer K., Klukas C., Altmann T., Sreenivasulu N., Bäumlein H., Kuhlmann M. AtRD22 and AtUSPL1, members of the plant-specific BURP domain family involved in Arabidopsis thaliana drought tolerance. PLoS ONE. 2014;9:e110065. doi: 10.1371/journal.pone.0110065. - DOI - PMC - PubMed

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