Alpha-synuclein seed amplification assay longitudinal outcomes in Lewy body disease spectrum

Abstract

Evidence from neuropathological cohorts indicates that a CSF α-synuclein (α-syn) seed amplification assay (SAA) might provide quantitative kinetic parameters correlating with α-syn pathology burden in patients with Lewy body disease (LBD). Studies are needed to assess their longitudinal trend during the presymptomatic and clinical disease phases and their correlation with measures of disease progression. We aimed to assess the baseline α-syn CSF SAA kinetic parameters, their longitudinal variations and associations with clinical outcomes in a longitudinal cohort of repeatedly sampled LBD patients, including clinically unimpaired (asymptomatic LBD) and neurologically impaired individuals. Participants from the prospective BioFINDER-1 study with longitudinal CSF collections (n = 718) were screened by α-syn SAA. CSF samples were tested in four replicates blinded to clinical diagnoses. The number of positive replicates (Nrep), the time needed by the fluorescence signal to reach the threshold (Lag) and the highest intensity of the fluorescent signal were analysed at baseline (time of first positive SAA) in all participants and longitudinally in those with at least two α-syn-positive CSF samples available. One hundred and ninety-six individuals (whole cohort) showing α-syn seeding activity were included. Of those, 170 participants tested positive by SAA in all available samples, while 26 converted from a negative to a positive test result during follow-up (LBD-converters), suggesting an early LBD stage. At baseline, LBD-converters showed lower Nrep (P = 0.001) and a longer Lag (P = 0.001) than subjects displaying α-syn seeding activity from the first available sample. The Nrep increased longitudinally in the whole cohort [β = 0.09, 95% confidence interval (95% CI) 0.06-0.12, P < 0.001], in asymptomatic LBD (β = 0.15, 95% CI 0.09-0.21, P < 0.001) and in Parkinson's disease individuals without dementia (β = 0.07, 95% CI 0.02-0.12, P = 0.01). The Lag decreased longitudinally in asymptomatic LBD (β = -0.24, 95% CI -0.42 to -0.06, P = 0.008). Baseline Nrep predicted the subsequent appearance of dementia in the whole cohort [hazard ratio (HR) 1.57, 95% CI 1.19-2.07, P = 0.001] and the Parkinson's disease subgroup (HR 1.83, 95% CI 1.17-2.85, P = 0.008). The difference between the Lag at each sampling and that at baseline was negatively associated with the appearance of dementia in the whole cohort (HR 0.76, 95% CI 0.59-0.99, P = 0.04) and in the Parkinson's disease subgroup (HR 0.69, 95% CI 0.50-0.95, P = 0.02). The α-syn SAA parameters Nrep and Lag showed associations with the LBD stage and the development of dementia. Furthermore, their longitudinal variation is coherent with progression of pathology over time. These data support the use of SAA kinetic parameters to monitor disease progression and therapeutic response.

Keywords: Lewy body disease; disease progression; kinetic parameters; longitudinal study; prion; seed amplification assay.

Figure 1

Comparison of baseline N_rep and Lag values between LBD-converters and non-LBD-converters and among different subgroups. (A–C) Values of N_rep (A), Lag_med1 (B) and Lag_med2 (C) in LBD-converters and non-LBD-converters. (D–F) Values of N_rep (D), Lag_med1 (E) and Lag_med2 (F) in participants belonging to different subgroups. Horizontal bold lines represent the median value, boxes represent the interquartile range, and whiskers represent the 5th and the 95th percentiles. Open circles represent the outliers. AD = Alzheimer’s disease; CI = cognitively impaired; CU = cognitively unimpaired; N_rep = number of positive replicates; LBD-c = Lewy body disease converters; non-LBD-c = non-Lewy body disease converters; PD = Parkinson’s disease; PDD = Parkinson’s disease dementia.

Figure 2

Longitudinal Lag variation in the asymptomatic LBD and the CI-AD subgroups. (A) Longitudinal trend of Lag_med2 in the asymptomatic LBD subgroup. (B) Longitudinal trend of Lag_med1 in the CI-AD subgroup. (C) Longitudinal trend of Lag_med2 in the CI-AD subgroup. Grey dotted lines represent the longitudinal variation at a single-patient level; thick lines represent the overall kinetic parameter longitudinal trend. AD = Alzheimer’s disease; CI = cognitively impaired; LBD = Lewy body disease.