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. 2025 Mar;47(3):321-329.
doi: 10.1007/s13258-024-01604-9. Epub 2024 Dec 18.

Dual-specificity phosphatase 23 functions as a promising prognostic biomarker in non-small cell lung cancer

Seula Keum  1 , Yoon Ji Lee  1 , Jung-Woong Kim  2 , Sangmyung Rhee  3
Affiliations

Dual-specificity phosphatase 23 functions as a promising prognostic biomarker in non-small cell lung cancer

Seula Keum et al. Genes Genomics. 2025 Mar.

Abstract

Background: The mechanical remodeling of tumor microenvironment is critical for non-small cell lung cancer (NSCLC) progression. Dual-specificity phosphatase 23 (DUSP23) has been previously identified as a mechano-responsive gene, but its role in NSCLC progression remains unknown.

Objective: We aim to elucidate the clinical significance of DUSP23 in NSCLC progression.

Methods: We analyzed the expression of DUSP23 in cancer using polyacrylamide hydrogels designed to mimic the stiffness of normal (soft; ~0.5 kPa) and cancerous (stiff; ~40 kPa) tissues. The prognostic significance of DUSP23 expression in patients was examined using public databases. Additionally, we conducted various cell-based assays and transcriptomic analyses in DUSP23-silenced NSCLC cell lines. A risk score prognosis model was constructed using univariate Cox regression and Kaplan-Meier analysis.

Results: Our findings show that DUSP23 is upregulated in stiff matrices and is highly associated with poor prognosis in patients with solid cancers such as NSCLC and breast cancer. Silencing of DUSP23 resulted in decreased cell proliferation and invasion. Transcriptomic profiling revealed that 182 genes were downregulated, and 230 genes upregulated following DUSP23-depletion. Notably, 182 downregulated genes were enriched in cancer-related pathways, including cell cycle progression and cytoskeleton organization. Through KEGG pathway analysis, we identified 11 cancer-related genes and developed a prognostic risk model. In this model, the high-risk group of NSCLC patients exhibited significantly shorter overall survival compared to low-risk group, based on public datasets.

Conclusion: Our study demonstrates the clinical significance of DUSP23 as a prognostic marker in NSCLC and highlights its potential role of DUSP23 in promoting NSCLC progression.

Keywords: Bioinformatic analysis; DUSP23; NSCLC; Prognostic biomarker; Transcriptome analysis.

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Conflict of interest statement

Declarations. Conflict of interest: Authors declare no conflicts of interest.

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References

    1. Aguilar BJ, Zhou H, Lu Q (2017) Cdc42 signaling pathway inhibition as a therapeutic target in ras- related cancers. Curr Med Chem 24:3485–3507 - PubMed - DOI
    1. Alonso A, Burkhalter S, Sasin J, Tautz L, Bogetz J, Huynh H, Bremer MC, Holsinger LJ, Godzik A, Mustelin T (2004a) The minimal essential core of a cysteine-based protein-tyrosine phosphatase revealed by a novel 16-kDa VH1-like phosphatase, VHZ. J Biol Chem 279:35768–35774 - PubMed - DOI
    1. Alonso A, Sasin J, Bottini N, Friedberg I, Friedberg I, Osterman A, Godzik A, Hunter T, Dixon J, Mustelin T (2004b) Protein tyrosine phosphatases in the human genome. Cell 117:699–711 - PubMed - DOI
    1. Altorki NK, Markowitz GJ, Gao D, Port JL, Saxena A, Stiles B, McGraw T, Mittal V (2019) The lung microenvironment: an important regulator of tumour growth and metastasis. Nat Rev Cancer 19:9–31 - PubMed - PMC - DOI
    1. Bargagli E, Bonti V, Ferrari K, Rosi E, Bindi A, Bartolucci M, Chiara M, Voltolini L (2017) Lung Cancer in patients with severe idiopathic pulmonary fibrosis: critical aspects. Vivo 31:773–777 - DOI

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