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. 2024 Dec 4:15:1474706.
doi: 10.3389/fphar.2024.1474706. eCollection 2024.

First-time report on compound isolation from two Colocasia species: vegetable-derived bioactive metabolites and their medicinal potential

Safaet Alam  1   2 , Fahmida Tasnim Richi  2 , Nazim Uddin Emon  3 , Abu Asad Chowdhury  2 , Choudhury Mahmood Hasan  2 , Mohammad Rashedul Haque  2
Affiliations

First-time report on compound isolation from two Colocasia species: vegetable-derived bioactive metabolites and their medicinal potential

Safaet Alam et al. Front Pharmacol. .

Erratum in

Abstract

Background: Colocasia affinis Schott and Colocasia gigantea Hook.f. are two commonly found vegetable species of the genus Colocasia, found mainly in the Asian region.

Objectives: The objective of this study was to isolate bioactive phytochemicals from C. affinis and C. gigantea and elucidate their structure employing the NMR technique followed by bioactivity evaluation.

Methodology: Column chromatography was utilized to isolate phytochemicals, followed by NMR analysis for characterization. An in vivo analgesic test was performed through an acetic acid-induced writhing test, an anti-inflammatory test was performed through a formalin-induced licking test, and an antidiarrheal test was performed through a castor oil-induced diarrhea model. The in vitro antimicrobial test was executed through the disc diffusion method. Computer-aided simulation was also implemented to validate the wet laboratory results.

Results: Six compounds from C. affinis and C. gigantea were isolated and characterized from the dichloromethane (DCM)-soluble fractions of the methanolic extracts of these two species. Three of the compounds were from C. gigantea and proposed as penduletin (C1), a mixture of α-amyrin (C2a), β-amyrin (C2b), and monoglyceride of stearic acid (C3), while the remaining three compounds were from C. affinis and proposed as penduletin (C4) (which was also isolated from C. gigantea), 7,8-(3″,3″-dimethyl-pyrano)-4'-hydroxy flavonol (C5), and lastly a mixture of 7,8-(3″,3″-dimethyl-pyrano)-4'-hydroxy flavonol (C5) and 4',7,8-trihydroxy flavonol (C6). These compounds showed promising zones of inhibition against Gram-positive and Gram-negative bacteria and fungi. In the antidiarrheal test, C5 demonstrated the highest reduction in castor oil-induced diarrhea (44.44%) at a dose of 20 mg/kg compared to loperamide's 77.78% reduction. However, the analgesic assessment showed a noteworthy inhibition of acetic acid-induced writhing by C1/C4 and C2 (56.52%) at a dose of 20 mg/kg compared to the 76.09% by diclofenac sodium. In comparison, C2 showed pronounced anti-inflammatory action by 68.15% and 52.06% reduction, respectively, in the early and later phases compared to the ibuprofen's outcomes of 73.54% and 74.68%. Plausible targets such as dihydrofolate reductase (DHFR) for antimicrobial, kappa opioid receptor (KOR) for antidiarrheal, and cyclooxygenase 2 (COX-2) for anti-inflammatory and analgesic activities showed a noteworthy binding affinity with isolated compounds, and ADME/T studies displayed these phytochemicals' drug-likeness profiles.

Conclusion: To the best of our knowledge, this is the first report on compound isolation from these plants, which demands further extensive research for more absolute findings.

Keywords: Colocasia affinis; Colocasia gigantea; NMR; analgesic; anti-inflammatory; antibacterial; antidiarrheal; vegetable.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Plant parts of C. gigantea and C. affinis.
FIGURE 2
FIGURE 2
Isolation and purity testing of phytochemicals employing thin layer chromatographic (TLC) techniques.
FIGURE 3
FIGURE 3
Structures of the identified phytocompounds from C. affinis, C. gigantea, and the standard drug molecules considered for the respective bioactivities.
FIGURE 4
FIGURE 4
1H-NMR spectrum of the compound penduletin isolated from C. gigantea and C. affinis.
FIGURE 5
FIGURE 5
1H-NMR spectrum of the compound mixture of α-amyrin and β-amyrin isolated from C. gigantea.
FIGURE 6
FIGURE 6
1H-NMR spectrum of the compound monoglyceride of stearic acid isolated from C. gigantea.
FIGURE 7
FIGURE 7
1H-NMR spectrum of the compound 7,8-(3″,3″-dimethyl-pyrano)-4′-hydroxy flavonol isolated from C. affinis.
FIGURE 8
FIGURE 8
1H-NMR spectrum of the compound mixture of 7,8-(3″,3″-dimethyl-pyrano)-4′-hydroxy flavonol and 4',7,8-trihydroxy flavonol isolated from C. affinis.
FIGURE 9
FIGURE 9
Graphical representation of the molecular interactions of the isolated phytocompounds with the DHFR enzyme with 3D and 2D visualization.
FIGURE 10
FIGURE 10
Graphical 3D and 2D visualization of the molecular interactions of the isolated phytocompounds with the KOR receptor.
FIGURE 11
FIGURE 11
Graphical 3D and 2D visualization of the molecular interactions of the isolated phytocompounds with the COX-2 enzyme.
FIGURE 12
FIGURE 12
Graphical 3D and 2D visualization of the molecular interactions of the standard drug molecules with their respective enzymes.
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