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. 2025:2894:43-51.
doi: 10.1007/978-1-0716-4342-6_5.

Induction on Insulin Resistance In Vitro

Eng Leng Saw  1
Affiliations

Induction on Insulin Resistance In Vitro

Eng Leng Saw. Methods Mol Biol. 2025.

Abstract

Cellular model serves as a crucial preclinical research tool, providing essential insights into the mechanistic aspects of disease biology. Particularly in the study of chronic metabolic disorders such as type 2 diabetes mellitus and obesity, palmitate (a saturated fatty acid) is often used as a key inducer of insulin resistance in vitro. Within this chapter, I delineate procedures aimed at inducing insulin resistance in AC16 human cardiac-derived cells. The method involves the strategic application of heightened concentrations of palmitate and glucose to specifically target the AKT/GLUT-4 signaling pathway.

Keywords: AC16 human cardiac-derived cells; AKT; GLUT-4; Glucose; Insulin resistance; Palmitate.

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