Recent developments in enlarging the scale of production of Plasmodium falciparum in vitro

Abstract

Several methods are described for the cultivation of Plasmodium falciparum. They include cultivation in (a) flow vessels that contain 12 ml of RBC suspension and are harvested three times a week, (b) a "tipper" that provides a similar yield, and (c) more recently, a large flat-bottomed vessel that holds 75 ml of suspension. Attempts are being made to develop techniques for synchronizing the cultures.