Methacrylated silk fibroin based composite hydrogel with ROS-scavenging and osteogenic ability to orchestrate diabetic bone regeneration

Abstract

The repair of diabetic bone defects is still filled with enormous challenges. Excessive reactive oxygen species (ROS) are regenerated in diabetic bone defect sites which is harmful to bone regeneration. Therefore, it's to a good strategy to scavenge the excess ROS to provide a friendly environment for diabetic bone defects repair. Herein, a novel composite hydrogel with ROS-scavenging and osteogenic ability is constructed. This methacrylated silk fibroin based composite hydrogel is capable of releasing tannin acid and inorganic ion, which can reduce oxidative stress, restore homeostasis and enhance osteogenesis. In vitro results indicated that the composite hydrogel could promote osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) under oxidative stress condition. Furthermore, in vivo results suggested that it can significantly promote bone regeneration in diabetic bone defects. In conclusion, this study provides critical insight into the biological mechanism and potential therapy for diabetic bone regeneration.

Keywords: Bone regeneration; Diabetes mellitus; Hydrogel; Oxidative stress; Silk fibroin; Tannin acid.