Screening and engineering of lycopene-producing strain Rhodococcus jostii for bio-upcycling of poly(ethylene terephthalate) waste

Abstract

Poly(ethylene terephthalate) (PET) is a widely used plastic, but its improper disposal has caused serious environmental pollution. The development of bioconversion for PET waste into high-value chemicals has gained significant attention as an innovative solution. In this study, a novel guided screening strategy involving mixed-bacteria fermentation and partitioned purification (MBF) was proposed to first successful isolate Rhodococcus jostii LETBE 8896, a strain capable of naturally producing 4 μg/L of lycopene from PET hydrolysate. Transcriptomic analysis identified the methylerythritol 4-phosphate (MEP) pathway as key to lycopene biosynthesis, with IspG identified as a critical regulatory enzyme. R. jostii with ispG overexpression enhanced lycopene production, with 819 μg/L in the simulated PET hydrolysate and 650 μg/L in the PET hydrolysate. Additionally, the use of butylated hydroxytoluene (BHT) as an antioxidant significantly improved lycopene production at 1 L scale level, achieving a maximum yield of 1865 μg/L with a molar conversion rate of 50.36 % from the PET hydrolysate, the highest reported for PET hydrolysate to date. These findings highlight the dual potential of R. jostii as a chassis strain for high-value chemical production and as a sustainable solution for PET upcycling. This study provides a novel approach to plastic waste management, contributing to the circular economy and global sustainability goals.

Keywords: Bioconversion; Lycopene; PET waste; Rhodococcus jostii; Upcycling.