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. 1985 Feb 8;833(2):303-7.
doi: 10.1016/0005-2760(85)90202-4.

Characterization of two molecular species GD3 ganglioside from bovine buttermilk

B Hauttecoeur, S Sonnino, R Ghidoni

Characterization of two molecular species GD3 ganglioside from bovine buttermilk

B Hauttecoeur et al. Biochim Biophys Acta. .

Abstract

Two gangliosides, representing 85% of total lipid-bound sialic acid, have been isolated from bovine buttermilk and characterized. Both contained long-chain base, glucose, galactose and sialic acid in the molar ratio 1:1:1:2, and gave, upon sialidase treatment, a neutral glycolipid, characterized as lactosylceramide. Partial acid hydrolysis, permethylation analysis and chromium trioxide oxidation indicated their basic oligosaccharide portion to be NeuAc alpha 2----8NeuAc alpha 2----3Gal beta 1----4Glc. The difference between the two forms was exclusively in the ceramide moiety of the molecule, one containing mainly long-chain (C22-C25) fatty acids and an equimolar proportion of C16 and C18 long-chain bases, and the other mainly palmitic acid and C18 long-chain base.

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