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. 2024 Dec 6:11:1432029.
doi: 10.3389/fsurg.2024.1432029. eCollection 2024.

An in vivo evaluation of the safety and efficacy of using decellularized bovine parietal peritoneum membranes as dural substitutes

Aidos Doskaliyev  1 , Vyacheslav Ogay  2 , Islambek Mussabekov  3 , Muratbek Satov  3 , Berik Zhetpisbayev  4 , Khalit Mustafin  5 , Xeniya Bobrova  6 , Raushan Auezova  5 , Serik Akshulakov  7
Affiliations

An in vivo evaluation of the safety and efficacy of using decellularized bovine parietal peritoneum membranes as dural substitutes

Aidos Doskaliyev et al. Front Surg. .

Abstract

Purpose: The reconstruction of dura matter is a challenging problem for neurosurgeons. A number of materials for dural reconstruction have recently been developed, but some of them have poor biocompatibility, poor mechanical properties, and adverse effects. Bovine parietal peritoneum is a promising natural material for regenerative medicine and reconstructive surgery. In this study, we conducted an in vivo evaluation of the safety and efficacy of using decellularized bovine peritoneum membranes (BPMs) as natural dural substitutes in a rabbit model.

Methods: The dural defects in mature New Zealand rabbits were studied. A BPM was sutured on the dural defect area of each animal. Autologous periosteum and collagen membranes (Lyoplant®) were used to facilitate a comparison with the BPMs. ELISA, histomorphological analysis, and hematological analysis were carried out to examine the safety and efficacy of using BPMs as dural substitutes.

Results: Our results showed that the BPMs demonstrated a deterioration rate that is suitable for gathering newly formed meningothelial tissue. The thickness and density of BPM fibers prevents resorption in the first few days after use as a plastic material, and the regeneration of the dura mater does not occur at an accelerated pace, meaning that the gradual formation of fibrous tissue prevents adhesion to the brain surface. It was observed that the BPM can integrate with the adjacent tissue to repair dural defects. Moreover, the transplantation of BPMs did not cause significant adverse effects or immunological responses, indicating the safety and good biocompatibility of the BPM.

Conclusion: Thus, our in vivo study in a rabbit model showed that decellularized BPMs may represent a biocompatible natural material that can be used in cases requiring dura matter repair without significant adverse effects.

Keywords: bovine parietal peritoneum; dura matter; dural substitute; duraplasty; regeneration.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
The surgical procedures of trepanation of the rabbit skull and transplantation of the materials for the plastic surgery of DM. (A) Trepanation of the skull using an electric drill spherical grooved and diamond tips, the trepanation area is depicted schematically in the lower left corner; (B) a burr hole after cranial trepanation; (C) dura matter defect; (D) the plastic material is fixed with sutures.
Figure 2
Figure 2
Representative gross and microscopic images of decellularized BPM. (A) Gross images of decellularized BPM. Scale bar 100 mm. (B) Representative image of decellularized BPM sectioned and stained with H&E. Scale bar 200 µm. (C) Representative transmission electron microscopic (TEM) image of collagen fibrils of BPM before decellularization. (D) TEM micrograph of collagen fibrils in decellularized BPM. Scale bar 200 nm.
Figure 3
Figure 3
DNA quantification of native and decellularized BPM samples.
Figure 4
Figure 4
Histological analysis of the implanted dura matter substitutes at day 7 after plastic surgery. Arrows show fibroblasts in the connective tissue membranes formula image and blood vessels formula image. (A–C,E–G,I–K) H&E staining; (D,H,L) Masson's trichrome (TRI) staining.
Figure 5
Figure 5
Histological analysis of the implanted dura matter substitutes at day 21 after plastic surgery. Arrows show fibroblasts in the connective tissue membranes formula image and blood vessels formula image. (A–C,E–G,I–K) H&E staining; (D,H,L) Masson's trichrome (TRI) staining.
Figure 6
Figure 6
Histological analysis of the implanted dura matter substitutes at day 63 after plastic surgery. Arrows show fibroblasts in the connective tissue membranes formula image and blood vessels formula image. (A–C,E–G,I–K) H&E staining; (D,H,L) Masson's trichrome (TRI) staining.
Figure 7
Figure 7
Number of infiltrated fibroblasts in BPM, autologous periosteum (AP) and collagen membrane lyoplant®, data are represented as the mean ± SD, (n = 7), *p < 0.05 compared to BPM group.
Figure 8
Figure 8
The leukocyte counts in peripheral blood after implantation of BPM, autologous periosteum (AP) and collagen membrane (lyoplant®), data are represented as the mean ± SD, (n = 7).
Figure 9
Figure 9
The levels of TNF-α and CRP in blood serum. Data are represented as the mean ± SD, (n = 5), *p < 0.05; **p < 0.01.
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