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. 2024 Dec 23;44(1):10.
doi: 10.1007/s00299-024-03405-6.

The role of sugar transporter BrSWEET11 in promoting plant early flowering and preliminary exploration of its molecular mechanism

Jingping Yuan  1   2 , Changwei Shen  3 , Xin Li  1   2 , Bo Sun  1   2 , Yu Xu  1   2 , Ruixiang Chen  1   2 , Chunhui Wu  1   2 , Nadeem Khan  4 , Xinlei Guo  1   2
Affiliations

The role of sugar transporter BrSWEET11 in promoting plant early flowering and preliminary exploration of its molecular mechanism

Jingping Yuan et al. Plant Cell Rep. .

Abstract

BrSWEET11 accelerated Arabidopsis thaliana flowering, while silencing Brsweet11 in Brassica rapa delayed flowering relative to controls. BrSWEET11 is involved in sucrose transport after being induced by long-day conditions. SWEETs (Sugars Will Eventually Be Exported Transporters) are sugar outflow transporters that may participate in the regulation of plant flowering. In this study, the open reading frame of Brassica rapa ssp. pekinensis SWEET11 (BrSWEET11) was cloned and found to be 858 bp in length and encode 285 amino acids, which is typical of SWEET family proteins. The BrSWEET11 gene was strongly expressed in reproductive growth organs, particularly flowers, according to tissue expression analyses and GUS histochemical staining. BrSWEET11 promotes early flowering in Arabidopsis thaliana by 3-4 days, whereas Brsweet11 silencing in Brassica rapa delays flowering by 8-12 days relative to controls. BrSWEET11 promoted early flowering in A. thaliana, and compared with that in control plants, flowering was delayed in Brsweet11-silenced Brassica rapa. Transcriptome analysis of BrSWEET11-overexpressing A. thaliana and wild-type (WT) plants was performed and the results showed that eight key flowering genes jointly regulated flowering time, which was also validated in the Brsweet11-silenced plants. In addition, through photoperiod treatments and sugar content measurements, it was found that the expression of BrSWEET11 is induced by long-day conditions and is involved in sucrose transport. Further investigation using yeast library screening, yeast two-hybrid, and bimolecular fluorescence complementation assay techniques revealed that the BrSWEET11 protein interacts with the sugar transporter 4a (BrSUT4a) protein. Therefore, BrSWEET11 was induced by long-day conditions, and may promote early flowering in Brassica rapa through sucrose transport. This study provides a theoretical basis for elucidating the molecular mechanism through which SWEET genes are involved in flowering time regulation in Brassica rapa.

Keywords: Brassica rapa; Flowering time; Protein interaction; Transcriptome sequencing; Viral-induced gene silencing.

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Conflict of interest statement

Declarations. Conflict of interest: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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