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. 2025 Apr;26(2):271-281.
doi: 10.1007/s40368-024-00962-y. Epub 2024 Dec 23.

Quantitative analysis of Streptococcus mutans, Bifidobacterium, and Scardovia Wiggsiae in occlusal biofilm and their association with Visible Occlusal Plaque Index (VOPI) and International Caries Detection and Assessment System (ICDAS)

P Thitisakyothin  1 , S Chanrat  2 , R L Srisatjaluk  3 , K Mitrakul  4
Affiliations

Quantitative analysis of Streptococcus mutans, Bifidobacterium, and Scardovia Wiggsiae in occlusal biofilm and their association with Visible Occlusal Plaque Index (VOPI) and International Caries Detection and Assessment System (ICDAS)

P Thitisakyothin et al. Eur Arch Paediatr Dent. 2025 Apr.

Abstract

Aims: To quantitatively detect S. mutans, Bifidobacterium, and S. wiggsiae in occlusal biofilm from permanent first molars based on the Visible Occlusal Plaque Index (VOPI), and to analyse the association between their levels and the occlusal enamel caries occurrence following the diagnosis of the International Caries Detection and Assessment System (ICDAS).

Study design: One hundred twenty plaque samples were collected from children aged 6-8 years and divided into four groups (n = 30 each group) according to VOPI scores (0 = no visible plaque, 1 = thin plaque, 2 = thick plaque, and 3 = heavy plaque). Scores 0 and 1 were identified by running dental probe on the groove. Scores 2 and 3 were visually identified. ICDAS scores were recorded by scoring 0-3 (0 = sound tooth surface, 1 = opacity or discoloration of enamel after air drying, 2 = visual change in enamel when wet, and 3 = localised enamel breakdown).

Methods: DNA was extracted from plaque samples and performed quantitative real-time PCR using SYBR green and specific primers for total bacteria including the 16S rRNA gene sequences conserved in all bacteria (BAC16S), S. mutans, Bifidobacterium, and S. wiggsiae.

Results: Ages of the children were different amongst VOPI groups (p < 0.001). Levels of total bacteria (p < 0.001) and S. mutans (p = 0.026) increased when VOPI increased. The ratio of S. mutans to total bacteria (p = 0.015) and the ratio of Bifidobacterium to total bacteria (p < 0.001) decreased from VOPI 0 to VOPI 3. Significant differences in total bacteria (p < 0.001) and S. mutans (p = 0.018) were detected from VOPI 0 to VOPI 2. A difference in Bifidobacterium (p < 0.001) was detected from VOPI 0 to VOPI 1.

Conclusion: Quantities of total bacteria (p < 0.001), S. mutans (p = 0.02) and ICDAS scores (p < 0.001) and VOPI scores were positively correlated. Quantities of ratio of S. mutans to total bacteria (p = 0.003) and ratio of Bifidobacterium to total bacteria (p < 0.001) and VOPI scores and ICDAS scores (p < 0.001) were negatively correlated.

Keywords: Bifidobacterium; S. mutans; S. wiggsiae; Occlusal biofilm; Real-time PCR.

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Conflict of interest statement

Declarations. Conflict of interest: All authors declare that he/she have no conflict of interest. Ethical approval: All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Informed consent: Informed consent was obtained from all individual participants included in the study.

Figures

Fig. 1
Fig. 1
Clinical pictures illustrating the VOPI scores (23)
Fig. 2
Fig. 2
Clinical pictures illustrating the ICDAS scores (26)
Fig. 3
Fig. 3
Agarose gel electrophoresis of real-time PCR product using universal primers BAC16S, and specific primers for S. mutans, Bifidobacterium and S. wiggsiae
See this image and copyright information in PMC

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