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. 2025:2884:305-332.
doi: 10.1007/978-1-0716-4298-6_19.

Multiplexed and Multiomic Mass Spectrometry Imaging of MALDI-IHC Photocleavable Mass Tag Tissue Probes, N-Glycomics, and the Extracellular Matrisome from FFPE Tissue Sections

Harrison B Taylor  1 , Jaclyn B Dunne  1 , Mark J Lim  2 , Gargey Yagnik  2 , Kenneth J Rothschild  2   3 , Anand Mehta  1 , Richard R Drake  1 , Peggi M Angel  4
Affiliations

Multiplexed and Multiomic Mass Spectrometry Imaging of MALDI-IHC Photocleavable Mass Tag Tissue Probes, N-Glycomics, and the Extracellular Matrisome from FFPE Tissue Sections

Harrison B Taylor et al. Methods Mol Biol. 2025.

Abstract

Recent work in single-cell imaging has allowed unprecedented insight into single-cell interactions that control disease progression. However, approaches to understanding the combined extracellular and cellular microenvironment are limited. In the current protocol, we describe an approach that allows single-cell type imaging using matrix-assisted laser desorption/ionization immunohistochemistry (MALDI-IHC) of UV (ultraviolet) photocleavable mass tags combined with N-glycomic and ECM-targeted proteomic imaging from the same formalin-fixed paraffin-embedded tissue section. These approaches use the same imaging mass spectrometry platform to provide a comprehensive view of both the cellular and extracellular tissue microenvironment.

Keywords: Antibody; Collagen; Collagen peptide imaging; Extracellular matrix; Fibrosis; Histology; Imaging mass spectrometry; MALDI-IHC; N-Glycan; N-Glycosylation; Photocleavable mass tags; Proteomics; Single-cell type; Tissue imaging.

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