Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025 Jan 4:103:skae388.
doi: 10.1093/jas/skae388.

Mogroside V protects lipopolysaccharides-induced lung inflammation chicken via suppressing inflammation mediated by the Th17 through the gut-lung axis

Yuan Li  1 , Kai Wang  1 , Dan Shen  1 , Junze Liu  1 , Sheng Li  1 , Luyao Liu  1 , Kentaro Nagaoka  2 , Chunmei Li  1
Affiliations

Mogroside V protects lipopolysaccharides-induced lung inflammation chicken via suppressing inflammation mediated by the Th17 through the gut-lung axis

Yuan Li et al. J Anim Sci. .

Abstract

Lipopolysaccharide (LPS) exposure triggers pulmonary inflammation, leading to compromised lung function in broiler. As amplified by policy restrictions on antibiotic usage, seeking antibiotic alternatives has become imperative. Mogroside V (MGV) has been reported to have a beneficial role in livestock and poultry production due to its remarkable antiinflammatory effects. Despite evidence showcasing MGV's efficacy against LPS-triggered lung inflammation, its precise mechanism of action remains elusive. In this study, we transplanted normal fecal microbiota (CF), fecal microbiota modified by MGV (MF), and sterile fecal filtrate (MS) into broiler with LPS-induced pneumonia. The results showed that through fecal microbiota transplantation (FMT), transplanting MGV-induced microbial populations significantly mitigated tissue damage induced by LPS and enhanced the mRNA level of pulmonary tight junction proteins and mucoprotein (P < 0.01). The expression levels of RORα (P < 0.001), Foxp3 (P < 0.01), and PD-L1 (P < 0.01) were significantly increased in the MF group than CF group. The concentrations of IL-6 and IL-17 in broilers lung tissue of MF group were lower than those in broilers of CF group (P < 0.05). Furthermore, the concentration of TGF-β in broilers serum of MS and MF groups was higher than those in broilers of CF group (P < 0.05). Microbial community analysis demonstrated that at genus level, the harmful bacterial populations Escherichia-Shigella and Helicobacter following FMT treatment were significantly reduced in MF group (P < 0.05), potentially mediating its protective effects. Compared with CF group, valerate content and FFAR2 mRNA expression levels in MF group were significantly increased (P < 0.05). The study suggests that MGV via the gut-lung axis, attenuates Th17-mediated inflammation, offering promise as a therapeutic strategy against LPS-induced lung inflammation in chickens.

Keywords: broiler; intestinal microbiota; lipopolysaccharides; mogroside V; pulmonary inflammation.

Plain language summary

Frequent respiratory problems during broiler breeding not only affect the growth performance of broilers, but also jeopardize the health and safety production of broilers in serious cases. Luo Han Guo is a traditional medicinal and food plant, and its extract mogroside V (MGV) has biological functions such as blood glucose lowering, bacteriostatic, antioxidant, and antiinflammator. MGV has a great potential for the treatment of respiratory diseases, but there are fewer studies on its application in broiler farming, especially in the protection of suction health of broiler chickens, which needs to be further investigated. Therefore, this experiment was designed to investigate the specific mechanism of MGV in relieving lung inflammation in broilers and to provide theoretical basis and corresponding countermeasures for the prevention of respiratory diseases in broilers. The results showed that dietary supplementation with MGV could promote the growth performance of broilers and alleviate lipopolysaccharide-induced acute lung injury in broilers by regulating the intestinal flora to enhance the barrier function of the lungs and inhibit the development of lung inflammation.

PubMed Disclaimer

Conflict of interest statement

The authors declare no real or perceived conflicts of interest.

Similar articles

See all similar articles

References

    1. Alvarado-Peña, N., Galeana-Cadena D., Gómez-García I. A., Mainero X. S., and Silva-Herzog E... 2023. The microbiome and the gut-lung axis in tuberculosis: interplay in the course of disease and treatment. Front. Microbiol. 14:1237998. doi: https://doi.org/10.3389/fmicb.2023.1237998 - DOI - PMC - PubMed
    1. Ansari, A. R., Ge X. H., Huang H. B., Huang X. Y., Zhao X., Peng K. M., Zhong J. M., and Liu H. Z... 2016. Effects of lipopolysaccharide on the histomorphology and expression of toll-like receptor 4 in the chicken trachea and lung. Avian Pathol. 45:530–537. doi: https://doi.org/10.1080/03079457.2016.1168923 - DOI - PubMed
    1. Budden, K. F., Gellatly S. L., Wood D. L., Cooper M. A., Morrison M., Hugenholtz P., and Hansbro P. M... 2017. Emerging pathogenic links between microbiota and the gut-lung axis. Nat. Rev. Microbiol. 15:55–63. doi: https://doi.org/10.1038/nrmicro.2016.142 - DOI - PubMed
    1. Chapman, M. E., Wang W., Erf G. F., and R. F.Wideman, Jr. 2005. Pulmonary hypertensive responses of broilers to bacterial lipopolysaccharide (LPS): evaluation of LPS source and dose, and impact of pre-existing pulmonary hypertension and cellulose micro-particle selection. Poult. Sci. 84:432–441. doi: https://doi.org/10.1093/ps/84.3.432 - DOI - PubMed
    1. Chen, C. M., Yang Y. S. H., Chou H. C., and Lin S... 2023. Intranasal administration of Lactobacillus johnsonii attenuates hyperoxia-induced lung injury by modulating gut microbiota in neonatal mice. J. Biomed. Sci. 30:57. doi: https://doi.org/10.1186/s12929-023-00958-8 - DOI - PMC - PubMed

MeSH terms

Substances