[Evaluation of Bacterial Identification Performance Using the I-dOne Measuring Device Based on the Principle of the ATR-FTIR Method]

Abstract

Recently, mass spectrometers based on MALDI-TOF MS, which can rapidly identify microorganisms, have become widely used, contributing to the proper use of antimicrobial agents and diagnosis of infectious diseases through rapid reporting of bacterial species. We had the opportunity to evaluate the performance of the I-dOne, a microorganism identification system using the ATR-FTIR method. A total of 174 strains, 78 of 11 Grampositive bacteria and 96 of 12 Gram-negative bacteria, registered in the I-dOne database were used. The strains were identified using the mass spectrometer MALDI Biotyper (Bruker Japan Inc.) and compared with the I-dOne identification results. The identification agreement rate was 73.3% (255 out of 348 shares), including Gram-positive bacteria and Gram-negative bacteria. I-dOne is expected to improve its identification accuracy through software updates and database additions and improvements.