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. 2024 Dec 8:2024:10.17912/micropub.biology.001398.
doi: 10.17912/micropub.biology.001398. eCollection 2024.

Complete annotated genome sequence of Microbacterium paraoxydans phage Evcara, a cluster GI podovirus isolated from compost

Hannah B Bonogafsky  1 , Kelly M Freer  1 , Taylor B Sanderson  1 , Kira A Yost  1 , Ayden J Pyle  1 , Ginavieve Rowley  1 , Maria D Gainey  1
Affiliations

Complete annotated genome sequence of Microbacterium paraoxydans phage Evcara, a cluster GI podovirus isolated from compost

Hannah B Bonogafsky et al. MicroPubl Biol. .

Abstract

Bacteriophage Evcara is a podovirus isolated on Microbacterium paraoxydans NRRL B-24275. Its genome is 16,285 bp in length and contains 22 predicted protein-coding genes. Evcara, has been assigned to cluster GI with Microbacterium foliorum phages PineapplePizza and Curie that share 10 homologues with the well-characterized Bacillus subtilis phage phi29.

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Conflict of interest statement

The authors declare that there are no conflicts of interest present.

Figures

Figure 1. Evcara plaque morphology and cluster GI genomic organization
Figure 1. Evcara plaque morphology and cluster GI genomic organization
(A) Evcara forms plaques with clear centers and two halos that vary in size (1mm to 6mm in diameter) and halo appearance when plated on M. paraoxydans PYCa top agar overlays incubated for 48hr at 30°C. (B) A representation of the genomes of all three cluster GI bacteriophages was generated using Phamerator. Genes are represented with numbered boxes, and assigned functions are listed. Boxes above and below the ruler represent genes that are transcribed rightwards and leftwards, respectively. PineapplePizza genes with phi29 homologues are denoted with a “*.” Genes represented as white boxes are genes with no homologues in the Actinobacteriophage database.  
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