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. 2024 Oct 28:13:105.
doi: 10.4103/abr.abr_341_24. eCollection 2024.

High Prevalence of Antimicrobial Resistance Genes in Multidrug-Resistant Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Clinical Isolates after COVID-19 Pandemic, North Iran

Golnar Rahimzadeh  1 , Shaghayegh Rezai  2 , Reza Valadan  3 , Raha Rezai  1 , Saman Soleimanpour  2 , Laleh Vahedi  4 , Somayeh Sheidaei  4 , Masoud Moradi  5 , Mohammad Sadegh Rezai  1 , Ebrahim Nemati  1
Affiliations

High Prevalence of Antimicrobial Resistance Genes in Multidrug-Resistant Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Clinical Isolates after COVID-19 Pandemic, North Iran

Golnar Rahimzadeh et al. Adv Biomed Res. .

Abstract

Background: Amid the COVID-19 pandemic, the surge in hospital admissions and widespread use of broad-spectrum antibiotics have heightened the risk of hospital-acquired infections from multidrug-resistant (MDR) organisms, particularly Escherichia coli. It is imperative to implement stringent measures to curb the spread of antimicrobial resistance in hospitals and devise robust treatment strategies for patients grappling with such infections. To confront this challenge, a comprehensive study was undertaken to examine MDR extended-spectrum beta-lactamase (MDR-ESBL)-producing Escherichia coli isolates from patients with nosocomial infections following the COVID-19 pandemic in Northern Iran.

Materials and methods: The current study was conducted as a cross-sectional study. A total of 12,834 samples were collected from patients with healthcare-associated infections at four designated corona centers in Northern Iran, following the COVID-19 pandemic. Antimicrobial resistance was determined using standard broth micro-dilution, while resistance genes were accurately detected using the multiplex PCR method.

Results: The results indicated that meropenem and ciprofloxacin had a resistance rate of 100% and 98.2%, respectively, while piperacillin-tazobactam showed the highest sensitivity rate at 54.4%. The frequency of specific genes, including bla IMP , bla TEM , AcrA, AcrB, bla CTX , bla OXA-58 , aaclb, bla SHV, and aacla, were found to be 100%, 100%, 99.1%, 99.1%, 91.2%, 80.7%, 64.9%, 44.7%, and 37.7%, respectively.

Conclusions: In the current study, over 50% of MDR-ESBL-producing Escherichia coli isolates exhibited resistance to antibiotics. A combination of antibiotics, including piperacillin-tazobactam and colistin, is recommended for treating extensively drug-resistant Escherichia coli infections.

Keywords: COVID-19; Escherichia coli; extended-spectrum beta-lactamase (ESBL); multidrug-resistant; multiplex polymerase chain reaction.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
Correlation between antibiotic resistance genes and susceptibility to antibiotics among MDR E. coli isolates. SAM: Ampicillin-Sulbactam, CIP: Ciprofloxacin, GM: Gentamicin, CL: Colistin, CAZ: Ceftazidime, MEN: Meropenem, CPM: Cefepime, PTZ: Piperacillin-Tazobactam
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References

    1. Rahimzadeh G, Valadan R, Rezai SH, Khosravi M, Vahedi Larijani L, Sheidaei S, et al. Evaluation of antibiotic resistance changes in Acinetobacter baumannii in the era of COVID-19 in Northern Iran. Iran J Microbiol. 2024;16:314–22. - PMC - PubMed
    1. Murray CJ, Ikuta KS, Sharara F, Swetschinski L, Robles Aguilar G, Gray A, et al. Global burden of bacterial antimicrobial resistance in 2019: A systematic analysis. Lancet. 2022;399:629–55. - PMC - PubMed
    1. Clermont O, Bonacorsi S, Bingen E. Rapid and simple determination of the Escherichia coli phylogenetic group. Appl Environ Microbiol. 2000;66:4555–8. - PMC - PubMed
    1. Galarce N, Arriagada G, Sánchez F, Venegas V, Cornejo J, Lapierre L. Antimicrobial use in companion animals: Assessing veterinarians’ prescription patterns through the first national survey in Chile. Animals (Basel) 2021;11:348–54. - PMC - PubMed
    1. World Health Organization Global priority list of antibiotic-resistant bacteria to guide research, discovery, and development of new antibiotics. Available from: https://www.who.int/news/item/27-02-2017-who-publishes-list-of-bacteria-... . [Last accessed on 2023 Oct 30]

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