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. 2024 Feb 1;3(1):2306766.
doi: 10.1080/27694127.2024.2306766. eCollection 2024 Dec 31.

TANGO2-related rhabdomyolysis symptoms are associated with abnormal autophagy functioning

Hortense de Calbiac #  1   2 , Sebastian Montealegre #  1 , Marjolène Straube  1   2 , Solène Renault  1   2 , Hugo Debruge  1   2 , Loïc Chentout  1   2 , Sorana Ciura  3 , Apolline Imbard  4 , Edouard Le Guillou  4 , Anca Marian  3 , Nicolas Goudin  5 , Laure Caccavelli  1   2 , Sylvie Fabrega  6 , Arnaud Hubas  7 , Peter van Endert  1   8 , Nicolas Dupont  1 , Julien Diana  1 , Edor Kabashi #  3 , Pascale de Lonlay #  1   2
Affiliations

TANGO2-related rhabdomyolysis symptoms are associated with abnormal autophagy functioning

Hortense de Calbiac et al. Autophagy Rep. .

Abstract

Patients with pathogenic variants in the TANGO2 gene suffer from severe and recurrent rhabdomyolysis episodes precipitated by fasting. Autophagy functioning was analyzed in vitro, in primary skeletal myoblasts from TANGO2 patients, in basal and fasting conditions, and TANGO2 mutations were associated with reduced LC3-II levels upon starvation. In zebrafish larvae, tango2 inhibition induced locomotor defects which were exacerbated by exposure to atorvastatin, a compound known to cause rhabdomyolysis. Importantly, rhabdomyolysis features of tango2 knockdown were associated with autophagy and mitophagy defects in zebrafish. Calpeptin treatment was sufficient to rescue the locomotor properties thanks to its beneficial effect on autophagy functioning in zebrafish and to improve LC3-II levels in starved primary muscle cells of TANGO2 patients. Overall, we demonstrated that TANGO2 plays an important role in autophagy thus giving rise to new therapeutic perspectives in the prevention of RM life-threatening episodes.

Keywords: Autophagy; TANGO2; calpeptin; myoblasts; rhabdomyolysis; zebrafish.

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Conflict of interest statement

No potential conflict of interest was reported by the author(s).

Figures

Figure 1.
Figure 1.
Autophagy is defective in TANGO2 patients’ primary myoblasts.
Figure 2.
Figure 2.
tango2 knockdown induces a RM-like phenotype in zebrafish due to autophagy defects.
Figure 3.
Figure 3.
Calpeptin ameliorates TANGO2 disease features in zebrafish and in primary myoblasts.
See this image and copyright information in PMC

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