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. 2024 Dec 11:15:1468577.
doi: 10.3389/fmicb.2024.1468577. eCollection 2024.

Improvement of silage characteristics of Lactobacillus salivarius HMC4 and improvement of silage quality of king grass

Jinsong Yang #  1 , Songsong Zhao #  1 , Wenbo Zhi #  1 , Tianyu Lu  1 , Huahua Qiao  1 , Wei Liu  1 , Ying Dou  1 , Haisheng Tan  2 , Hanlin Zhou  3   4
Affiliations

Improvement of silage characteristics of Lactobacillus salivarius HMC4 and improvement of silage quality of king grass

Jinsong Yang et al. Front Microbiol. .

Abstract

The effect of HMC4 produced by protoplast fusion on silage was studied. The silage formula was composed of heterozygote HMC4 (Group C), parent Lactobacillus (Group A) and a combination of two parents (Group B). The fermentation quality and microbial composition of each batch of silage were evaluated. The results showed that the propionic acid concentration in group C was the lowest, but the organic acid content in feed was significantly increased. Groups B and C had slightly lower crude fiber levels than group A, while groups A and C had higher levels of soluble sugars. The dynamic observation of C group showed that the nutrient composition of feed changed gradually with the extension of silage period. High-throughput sequencing revealed fluctuations in microbial composition before and after silage. Over time, Lactobacillus became the dominant strain and its numbers steadily increased.

Keywords: Lactobacillus; high-throughput sequencing; microbial communities; protoplast fusion; silage.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Standard curve of sucrose.
Figure 2
Figure 2
Sequencing assembly results. Panel (a–c) are the abundance curve, dilution curve, and petal diagram after sequence assembly under direct open bag grouping; (d–f) are the abundance curve, dilution curve, and petal diagram under dynamic open bag grouping.
Figure 3
Figure 3
NMDS analysis with different additives (a) and different silage times (b).
Figure 4
Figure 4
Phylum level species stacking plots for different additives (a) and different silage times (b).
Figure 5
Figure 5
Genus level species stacking plots for different additives (a) and different silage times (b).
Figure 6
Figure 6
Functional clustering analysis of different additives.
Figure 7
Figure 7
Functional clustering analysis of different silage times.
See this image and copyright information in PMC

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