Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Dec 11:15:1468577.
doi: 10.3389/fmicb.2024.1468577. eCollection 2024.

Improvement of silage characteristics of Lactobacillus salivarius HMC4 and improvement of silage quality of king grass

Jinsong Yang #  1 , Songsong Zhao #  1 , Wenbo Zhi #  1 , Tianyu Lu  1 , Huahua Qiao  1 , Wei Liu  1 , Ying Dou  1 , Haisheng Tan  2 , Hanlin Zhou  3   4
Affiliations

Improvement of silage characteristics of Lactobacillus salivarius HMC4 and improvement of silage quality of king grass

Jinsong Yang et al. Front Microbiol. .

Abstract

The effect of HMC4 produced by protoplast fusion on silage was studied. The silage formula was composed of heterozygote HMC4 (Group C), parent Lactobacillus (Group A) and a combination of two parents (Group B). The fermentation quality and microbial composition of each batch of silage were evaluated. The results showed that the propionic acid concentration in group C was the lowest, but the organic acid content in feed was significantly increased. Groups B and C had slightly lower crude fiber levels than group A, while groups A and C had higher levels of soluble sugars. The dynamic observation of C group showed that the nutrient composition of feed changed gradually with the extension of silage period. High-throughput sequencing revealed fluctuations in microbial composition before and after silage. Over time, Lactobacillus became the dominant strain and its numbers steadily increased.

Keywords: Lactobacillus; high-throughput sequencing; microbial communities; protoplast fusion; silage.

PubMed Disclaimer

Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Standard curve of sucrose.
Figure 2
Figure 2
Sequencing assembly results. Panel (a–c) are the abundance curve, dilution curve, and petal diagram after sequence assembly under direct open bag grouping; (d–f) are the abundance curve, dilution curve, and petal diagram under dynamic open bag grouping.
Figure 3
Figure 3
NMDS analysis with different additives (a) and different silage times (b).
Figure 4
Figure 4
Phylum level species stacking plots for different additives (a) and different silage times (b).
Figure 5
Figure 5
Genus level species stacking plots for different additives (a) and different silage times (b).
Figure 6
Figure 6
Functional clustering analysis of different additives.
Figure 7
Figure 7
Functional clustering analysis of different silage times.
See this image and copyright information in PMC

References

    1. Aleksandrzak-Piekarczyk T., Stasiak-Różańska L., Cieśla J., Bardowski J. (2014). ClaR—a novel key regulator of cellobiose and lactose metabolism in Lactococcus lactis IL1403. Applied Microbiology and Biotechnology 99, 337–347. doi: 10.1007/s00253-014-6067-y - DOI - PMC - PubMed
    1. Ali N., Wang S., Zhao J., Dong Z., Li J., Nazar M., et al. (2020). Microbial diversity and fermentation profile of red clover silage inoculated with reconstituted indigenous and exogenous epiphytic microbiota. Bioresour. Technol. 314:123606. doi: 10.1016/j.biortech.2020.123606, PMID: - DOI - PubMed
    1. Cardona E., Rios J., Peña J., Peñuela M., Rios L. (2016). King grass: a very promising material for the production of second generation ethanol in tropical countries. Biomass Bioenergy 95, 206–213. doi: 10.1016/j.biombioe.2016.10.008 - DOI
    1. Chen R., Li M., Yang J., Chen L., Zi X., Zhou H., et al. (2022). Exploring the effect of wilting on fermentation profiles and microbial community structure during ensiling and air exposure of king grass silage. Front. Microbiol. 13:971426. doi: 10.3389/fmicb.2022.971426 - DOI - PMC - PubMed
    1. Davey M. R., Anthony P., Power J. B., Lowe K. C. (2005). Plant protoplasts: status and biotechnological perspectives. Biotechnology Advances 23, 131–171. doi: 10.1016/j.biotechadv.2004.09.008 - DOI - PubMed

LinkOut - more resources