Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Dec 17:111:129-146.
doi: 10.3897/mycokeys.111.136922. eCollection 2024.

Multigene phylogeny, taxonomy, and potential biological properties of Pseudoroussoella and Neoroussoella species (Roussoellaceae, Dothideomycetes) from Asteraceae weeds in northern Thailand

Zin Hnin Htet  1 , Kevin D Hyde  1   2   3 , Fatimah O Alotibi  3 , Thilini K W Chethana  1 , Ausana Mapook  1
Affiliations

Multigene phylogeny, taxonomy, and potential biological properties of Pseudoroussoella and Neoroussoella species (Roussoellaceae, Dothideomycetes) from Asteraceae weeds in northern Thailand

Zin Hnin Htet et al. MycoKeys. .

Abstract

In our study, dead stems of two Asteraceae species (weeds) were collected from northern Thailand. Both morphology and multigene phylogeny were used to determine the identity of the taxa. Maximum likelihood and Bayesian inference analyses of combined LSU, SSU, ITS, tef1-α and rpb2 data revealed two new species Pseudoroussoellabidenticola, and Neoroussoellachromolaenae with one new host record of N.entadae. Preliminary investigation into antibacterial properties revealed that our three isolates inhibited the growth of Bacillussubtilis, Escherichiacoli, and Staphylococcusaureus. Additionally, we present updated phylogenetic trees for Roussoellaceae, accompanied by detailed descriptions and illustrations of the three identified species.

Keywords: 2 new species; Ascomycota; Bidenspilosa; Chromolaenaodorata; antibacterial properties; new host record.

PubMed Disclaimer

Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Figure 1.
Figure 1.
Phylogram generated from maximum likelihood analysis based on the combined dataset of LSU, SSU, ITS, tef1-α and rpb2 sequence data. Bootstrap support values for ML equal to or greater than 75% and BYPP equal to or greater than 0.90 are given at the nodes. Newly generated sequences are in blue and type species are in bold.
Figure 2.
Figure 2.
Neoroussoellachromolaenae (MFLU 24-0264, holotype) a, b Conidiomata on the substrate c a section through conidioma d ostiole e peridium f conidia and conidiogenous cells g–i conidia j germinating conidia k culture on the MEA. Scale bars: 500 µm (a, b); 100 µm (c); 20 µm (d, e); 10 µm (e–j).
Figure 3.
Figure 3.
Neoroussoellaentadae (MFLU 24-0265, new host record) a, b Conidiomata on the substrate c a section through conidioma d ostiole e peridium f conidia and conidiogenous cells g–i conidia j a germinating conidium k culture on the MEA. Scale bars: 500 µm (a); 200 µm (b); 50 µm (c); 20 µm (d, e); 5 µm (f, g, h, i, j).
Figure 4.
Figure 4.
Pseudoroussoellabidenticola (MFLU 24-0266, holotype) a, b Conidiomata on the substrate c a section through a conidioma d peridium e conidia and conidiogenous cell f–i conidia j germinating conidia k culture on the MEA. Scale bars: 500 µm (a, b); 100 µm (c); 10 µm (d); 5 µm (e–i).
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Abdou R, Scherlach K, Dahse HM, Sattler I, Hertweck C. (2010) Botryorhodines A-D, antifungal and cytotoxic depsidones from Botryosphaeriarhodina, an endophyte of the medicinal plant Bidenspilosa. Phytochemistry 71(1): 110–116. 10.1016/j.phytochem.2009.09.024 - DOI - PubMed
    1. Ahmed SA, Stevens DA, van de Sande WW, Meis JF, De Hoog GS. (2014) Roussoellapercutanea, a novel opportunistic pathogen causing subcutaneous mycoses. Medical Mycology 52(7): 689–698. 10.1093/mmy/myu035 - DOI - PubMed
    1. Alam ST, Le TAN, Park JS, Kwon HC, Kang K. (2019) Antimicrobial biophotonic treatment of ampicillin-resistant Pseudomonasaeruginosa with hypericin and ampicillin cotreatment followed by orange light. Pharmaceutics 11(12): 641. 10.3390/pharmaceutics11120641 - DOI - PMC - PubMed
    1. Balouiri M, Sadiki M, Ibnsouda SK. (2016) Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis 6(2): 71–79. 10.1016/j.jpha.2015.11.005 - DOI - PMC - PubMed
    1. Chaiwan N, Gomdola D, Wang S, Monkai J, Tibpromma S, Doilom M, Wanasinghe DN, Mortimer PE, Lumyong S, Hyde KD. (2021) An online database providing updated information of microfungi in the Greater Mekong Subregion. Mycosphere 12(1): 1513–1526. 10.5943/mycosphere/12/1/19 - DOI

LinkOut - more resources