. 2025 Jan 20;8(1):399-409.

doi: 10.1021/acsabm.4c01373. Epub 2024 Dec 26.

Platelet Membrane-Coated HGF-PLGA Nanoparticles Promote Therapeutic Angiogenesis and Tissue Perfusion Recovery in Ischemic Hindlimbs

Peng Wang¹, Xiao Di¹, Fengshi Li², Zhihua Rong³, Wenzhuo Lian¹, Zongshu Li⁴, Tianqi Chen⁴, Wenjing Wang⁵, Qing Zhong⁴, Guoqiang Sun⁶, Leng Ni¹, ChangWei Liu¹

Affiliations

¹ Department of Vascular Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, No. 1 Shuaifuyuan Hutong, Dongcheng District, Beijing 100730, China.
² Department of Vascular Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
³ Department of Vascular Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.
⁴ Biomedical Engineering Facility of National Infrastructures for Translational Medicine, Institute of Clinical Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
⁵ Laboratory Animal Research Facility, National Infrastructures for Translational Medicine, Institute of Clinical Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100730, China.
⁶ Department of Information Center, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, No. 1 Shuaifuyuan Hutong, Dongcheng District, Beijing 100730, China.

PMID: 39723905
PMCID: PMC11753260 (available on 2025-12-26)
DOI: 10.1021/acsabm.4c01373

Platelet Membrane-Coated HGF-PLGA Nanoparticles Promote Therapeutic Angiogenesis and Tissue Perfusion Recovery in Ischemic Hindlimbs

Peng Wang et al. ACS Appl Bio Mater. 2025.

. 2025 Jan 20;8(1):399-409.

doi: 10.1021/acsabm.4c01373. Epub 2024 Dec 26.

Authors

Peng Wang¹, Xiao Di¹, Fengshi Li², Zhihua Rong³, Wenzhuo Lian¹, Zongshu Li⁴, Tianqi Chen⁴, Wenjing Wang⁵, Qing Zhong⁴, Guoqiang Sun⁶, Leng Ni¹, ChangWei Liu¹

Affiliations

¹ Department of Vascular Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, No. 1 Shuaifuyuan Hutong, Dongcheng District, Beijing 100730, China.
² Department of Vascular Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
³ Department of Vascular Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.
⁴ Biomedical Engineering Facility of National Infrastructures for Translational Medicine, Institute of Clinical Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
⁵ Laboratory Animal Research Facility, National Infrastructures for Translational Medicine, Institute of Clinical Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100730, China.
⁶ Department of Information Center, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, No. 1 Shuaifuyuan Hutong, Dongcheng District, Beijing 100730, China.

PMID: 39723905
PMCID: PMC11753260 (available on 2025-12-26)
DOI: 10.1021/acsabm.4c01373

Abstract

Therapeutic angiogenesis has garnered significant attention as a potential treatment strategy for lower limb ischemic diseases. Although hepatocyte growth factor (HGF) has been identified as a key promoter of therapeutic angiogenesis, its clinical application is limited due to its short half-life. In this study, we successfully developed and characterized platelet membrane-coated HGF-poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs). These nanoparticles demonstrated enhanced capabilities to promote endothelial cell (EC) proliferation, migration, and tube formation in vitro. Additionally, their efficacy in improving tissue perfusion and promoting angiogenesis was confirmed in a hindlimb ischemia rat model. Our findings suggest that platelet membrane-coated HGF-PLGA-NPs could serve as a promising therapeutic approach for enhancing angiogenesis and restoring tissue perfusion in ischemic conditions.

Keywords: chronic limb-threatening ischemia; drug delivery; hepatocyte growth factor; peripheral artery disease; therapeutic angiogenesis.

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Conflict of interest statement

The authors declare no competing financial interest.

Cited by

Glycometabolic Regulation of Angiogenesis: Mechanisms and Therapeutic Strategies.
Yao Z, Li J, Yu J, Cheng Y, Fang C, Chen X, Chen X, Wang Y, Gao D, Lin F. Yao Z, et al. Int J Mol Sci. 2025 Mar 7;26(6):2386. doi: 10.3390/ijms26062386. Int J Mol Sci. 2025. PMID: 40141029 Free PMC article. Review.

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Platelet Membrane-Coated HGF-PLGA Nanoparticles Promote Therapeutic Angiogenesis and Tissue Perfusion Recovery in Ischemic Hindlimbs

Affiliations

Platelet Membrane-Coated HGF-PLGA Nanoparticles Promote Therapeutic Angiogenesis and Tissue Perfusion Recovery in Ischemic Hindlimbs

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