A Novel Targeted Long-read Sequencing Approach Boosts Transcriptomic Profiling

doi:10.1093/gpbjnl/qzae090

. 2025 Jan 15;22(6):qzae090.

doi: 10.1093/gpbjnl/qzae090.

A Novel Targeted Long-read Sequencing Approach Boosts Transcriptomic Profiling

Xiaolong Tian^{1

2}, Rong Fan^{2

3

4

5}

Affiliations

¹ Department of Genetics, Yale School of Medicine, New Haven, CT 06520, USA.
² Department of Biomedical Engineering, Yale University, New Haven, CT 06520, USA.
³ Yale Stem Cell Center and Yale Cancer Center, Yale School of Medicine, New Haven, CT 06520, USA.
⁴ Department of Pathology, Yale School of Medicine, New Haven, CT 06520, USA.
⁵ Human and Translational Immunology, Yale School of Medicine, New Haven, CT 06520, USA.

PMID: 39724297
PMCID: PMC11802469
DOI: 10.1093/gpbjnl/qzae090

A Novel Targeted Long-read Sequencing Approach Boosts Transcriptomic Profiling

Xiaolong Tian et al. Genomics Proteomics Bioinformatics. 2025.

. 2025 Jan 15;22(6):qzae090.

doi: 10.1093/gpbjnl/qzae090.

Authors

Xiaolong Tian^{1

2}, Rong Fan^{2

3

4

5}

Affiliations

¹ Department of Genetics, Yale School of Medicine, New Haven, CT 06520, USA.
² Department of Biomedical Engineering, Yale University, New Haven, CT 06520, USA.
³ Yale Stem Cell Center and Yale Cancer Center, Yale School of Medicine, New Haven, CT 06520, USA.
⁴ Department of Pathology, Yale School of Medicine, New Haven, CT 06520, USA.
⁵ Human and Translational Immunology, Yale School of Medicine, New Haven, CT 06520, USA.

PMID: 39724297
PMCID: PMC11802469
DOI: 10.1093/gpbjnl/qzae090

No abstract available

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Conflict of interest statement

RF is scientific founder and advisor of IsoPlexis, Singleron Biotechnologies and AtlasXomics. The interests of RF were reviewed and managed by Yale University Provost’s Office in accordance with the university’s conflict of interest policies. XT declares no competing interests.

References

1. Wang F, Xu Y, Wang R, Zhang B, Smith N, Notaro A, et al. TEQUILA-seq: a versatile and low-cost method for targeted long-read RNA sequencing. Nat Commun 2023;14:4760. - PMC - PubMed
1. Liu Y, Yang M, Deng Y, Su G, Enninful A, Guo CC, et al. High-spatial-resolution multi-omics sequencing via deterministic barcoding in tissue. Cell 2020;183:1665–81. - PMC - PubMed
1. Deng Y, Bartosovic M, Kukanja P, Zhang D, Liu Y, Su G, et al. Spatial-CUT&Tag: spatially resolved chromatin modification profiling at the cellular level. Science 2022;375:681–6. - PMC - PubMed
1. Deng Y, Bartosovic M, Ma S, Zhang D, Kukanja P, Xiao Y, et al. Spatial profiling of chromatin accessibility in mouse and human tissues. Nature 2022;609:375–83. - PMC - PubMed
1. Liu Y, DiStasio M, Su G, Asashima H, Enninful A, Qin X, et al. High-plex protein and whole transcriptome co-mapping at cellular resolution with spatial CITE-seq. Nat Biotechnol 2023;41:1405–9. - PMC - PubMed

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[1] Wang F, Xu Y, Wang R, Zhang B, Smith N, Notaro A, et al. TEQUILA-seq: a versatile and low-cost method for targeted long-read RNA sequencing. Nat Commun 2023;14:4760. - PMC - PubMed

[2] Wang F, Xu Y, Wang R, Zhang B, Smith N, Notaro A, et al. TEQUILA-seq: a versatile and low-cost method for targeted long-read RNA sequencing. Nat Commun 2023;14:4760. - PMC - PubMed

[3] Liu Y, Yang M, Deng Y, Su G, Enninful A, Guo CC, et al. High-spatial-resolution multi-omics sequencing via deterministic barcoding in tissue. Cell 2020;183:1665–81. - PMC - PubMed

[4] Liu Y, Yang M, Deng Y, Su G, Enninful A, Guo CC, et al. High-spatial-resolution multi-omics sequencing via deterministic barcoding in tissue. Cell 2020;183:1665–81. - PMC - PubMed

[5] Deng Y, Bartosovic M, Kukanja P, Zhang D, Liu Y, Su G, et al. Spatial-CUT&Tag: spatially resolved chromatin modification profiling at the cellular level. Science 2022;375:681–6. - PMC - PubMed

[6] Deng Y, Bartosovic M, Kukanja P, Zhang D, Liu Y, Su G, et al. Spatial-CUT&Tag: spatially resolved chromatin modification profiling at the cellular level. Science 2022;375:681–6. - PMC - PubMed

[7] Deng Y, Bartosovic M, Ma S, Zhang D, Kukanja P, Xiao Y, et al. Spatial profiling of chromatin accessibility in mouse and human tissues. Nature 2022;609:375–83. - PMC - PubMed

[8] Deng Y, Bartosovic M, Ma S, Zhang D, Kukanja P, Xiao Y, et al. Spatial profiling of chromatin accessibility in mouse and human tissues. Nature 2022;609:375–83. - PMC - PubMed

[9] Liu Y, DiStasio M, Su G, Asashima H, Enninful A, Qin X, et al. High-plex protein and whole transcriptome co-mapping at cellular resolution with spatial CITE-seq. Nat Biotechnol 2023;41:1405–9. - PMC - PubMed

[10] Liu Y, DiStasio M, Su G, Asashima H, Enninful A, Qin X, et al. High-plex protein and whole transcriptome co-mapping at cellular resolution with spatial CITE-seq. Nat Biotechnol 2023;41:1405–9. - PMC - PubMed

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A Novel Targeted Long-read Sequencing Approach Boosts Transcriptomic Profiling

Affiliations

A Novel Targeted Long-read Sequencing Approach Boosts Transcriptomic Profiling

Authors

Affiliations

Conflict of interest statement

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