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. 1985 Mar;47(3):827-30.
doi: 10.1128/iai.47.3.827-830.1985.

Selective detection of Mycoplasma hyorhinis using cloned genomic DNA fragments

M A Taylor, K S Wise, M A McIntosh

Selective detection of Mycoplasma hyorhinis using cloned genomic DNA fragments

M A Taylor et al. Infect Immun. 1985 Mar.

Abstract

A 13-kilobase DNA fragment from a genomic library of Mycoplasma hyorhinis demonstrated specific Southern hybridization and dot hybridization when tested against a group of different mycoplasmas. This probe selectively recognized M. hyorhinis sequences in purified DNA, broth-grown organisms, and infected cell cultures, providing a direct method for differential detection of this species.

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